KO-Validated Antibodies

Primary Antibodies

KO-Validated Antibodies

Knockout (KO) validation is one of the most trusted ways to check an antibody works as intended. Each antibody in this range has been tested against a model where the target gene has been switched off. No signal in that knockout sample means the antibody is picking up your protein of interest and not something else.

Browse all KO-Validated antibodies

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Rabbit (582) Mouse (159) Rat (1)
About KO validation Why choose KO-validated Clonality
Product overview

What are KO-validated antibodies?

St John's Laboratory lists 743 KO-validated antibodies, tested against knockout cell lines to confirm specificity and make results easier to reproduce.

  • Tested against knockout models Each antibody has been tested where the protein coding gene has been fully switched off.
  • Confirms specificity No signal in the knockout sample confirms the antibody is detecting your target and not another protein.
  • Works across applications Suited to Western blotting, immunohistochemistry (IHC), flow cytometry and other common applications.
Why choose KO-validated

Built for reliable, reproducible data

  • High specificity Tested against knockout models to reduce cross-reactivity and false positives.
  • Consistent performance Built to perform the same way across repeat experiments.
  • Less time troubleshooting Spend less time chasing unclear results and more time on your own data.
Clonality

Monoclonal and polyclonal options

Monoclonal options give you a single, consistent epitope for repeat experiments, while polyclonal options bind multiple epitopes on the target for stronger signal detection.

Monoclonal (392) Polyclonal (351)

Need help choosing a KO-validated antibody?

Contact our technical support team for help finding an antibody for your target of interest. Each product page includes a downloadable datasheet and SDS, with dispatch on overnight priority shipping with gel ice packs.

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