![Western blot analysis of various lysates, using [KO Validated] SIRT5 rabbit polyclonal antibody (STJ28350) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/101643/389632/STJ28350_WB_2__71470.1713159351.jpg?c=1 "Western blot analysis of various lysates, using [KO Validated] SIRT5 rabbit polyclonal antibody (STJ28350) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.")
Anti-SIRT5 antibody (37-310) (STJ28350)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SIRT5 (37-310) antibody for use in WB and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SIRT5 |
| Gene ID | 23408 |
| Uniprot ID | SIR5_HUMAN |
| Immunogen Region | 37-310 |
| Immunogen Sequence | SSSMADFRKFFAKAKHIVII SGAGVSAESGVPTFRGAGGY WRKWQAQDLATPLAFAHNPS RVWEFYHYRREVMGSKEPNA GHRAIAECETRLGKQGRRVV VITQNIDELHRKAGTKNLLE IHGSLFKTRCTSCGVVAENY KSPICPALSGKGAPEPGTQD ASIPVEKLPRCEEAGCGGLL RPHVVWFGENLDPAILEEVD RELAHCDLCLVVGTSSVVYP AAMFAPQVAARGVPVAEFN |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 37-310 of human SIRT5 (NP_036373.1). |
Additional Info
| Tissue Specificity | Widely expressed. |
| Function | NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. Has weak NAD-dependent protein deacetylase activity.however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX. |
| Protein Name | Nad-Dependent Protein Deacylase Sirtuin-5 - MitochondrialRegulatory Protein Sir2 Homolog 5Sir2-Like Protein 5 |
| Database Links | Reactome: R-HSA-2151201 |
| Cellular Localisation | Mitochondrion MatrixMitochondrion Intermembrane SpaceCytoplasmCytosolNucleusMainly MitochondrialAlso Present ExtramitochondriallyWith A Fraction Present In The Cytosol And Very Small Amounts Also Detected In The NucleusIsoform 1: CytoplasmMitochondrionIsoform 2: Mitochondrion |
| Alternative Antibody Names | Anti-Nad-Dependent Protein Deacylase Sirtuin-5 - Mitochondrial antibodyAnti-Regulatory Protein Sir2 Homolog 5 antibodyAnti-Sir2-Like Protein 5 antibodyAnti-SIRT5 antibodyAnti-SIR2L5 antibody |
Information sourced from Uniprot.org