• Western blot analysis of extracts from normal (control) and SIRT5 knockout (KO) 293T cells, using SIRT5 antibody (STJ28350) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates, using [KO Validated] SIRT5 rabbit polyclonal antibody (STJ28350) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Anti-SIRT5 antibody (37-310) (STJ28350)

Anti-SIRT5 antibody (37-310) (STJ28350)

SKU:
STJ28350

£212.50 - £501.50
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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SIRT5 (37-310) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SIRT5
Gene ID: 23408
Uniprot ID: SIR5_HUMAN
Immunogen Region: 37-310
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 37-310 of human SIRT5 (NP_036373.1).
Immunogen Sequence: SSSMADFRKFFAKAKHIVII SGAGVSAESGVPTFRGAGGY WRKWQAQDLATPLAFAHNPS RVWEFYHYRREVMGSKEPNA GHRAIAECETRLGKQGRRVV VITQNIDELHRKAGTKNLLE IHGSLFKTRCTSCGVVAENY KSPICPALSGKGAPEPGTQD ASIPVEKLPRCEEAGCGGLL RPHVVWFGENLDPAILEEVD RELAHCDLCLVVGTSSVVYP AAMFAPQVAARGVPVAEFN
Tissue Specificity Widely expressed.
Function NAD-dependent lysine demalonylase, desuccinylase and deglutarylase that specifically removes malonyl, succinyl and glutaryl groups on target proteins. Activates CPS1 and contributes to the regulation of blood ammonia levels during prolonged fasting: acts by mediating desuccinylation and deglutarylation of CPS1, thereby increasing CPS1 activity in response to elevated NAD levels during fasting. Activates SOD1 by mediating its desuccinylation, leading to reduced reactive oxygen species. Activates SHMT2 by mediating its desuccinylation. Modulates ketogenesis through the desuccinylation and activation of HMGCS2. Has weak NAD-dependent protein deacetylase activity.however this activity may not be physiologically relevant in vivo. Can deacetylate cytochrome c (CYCS) and a number of other proteins in vitro such as UOX.
Protein Name Nad-Dependent Protein Deacylase Sirtuin-5 - Mitochondrial
Regulatory Protein Sir2 Homolog 5
Sir2-Like Protein 5
Database Links Reactome: R-HSA-2151201
Cellular Localisation Mitochondrion Matrix
Mitochondrion Intermembrane Space
Cytoplasm
Cytosol
Nucleus
Mainly Mitochondrial
Also Present Extramitochondrially
With A Fraction Present In The Cytosol And Very Small Amounts Also Detected In The Nucleus
Isoform 1: Cytoplasm
Mitochondrion
Isoform 2: Mitochondrion
Alternative Antibody Names Anti-Nad-Dependent Protein Deacylase Sirtuin-5 - Mitochondrial antibody
Anti-Regulatory Protein Sir2 Homolog 5 antibody
Anti-Sir2-Like Protein 5 antibody
Anti-SIRT5 antibody
Anti-SIR2L5 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance