• Immunohistochemistry analysis of paraffin-embedded mouse spleen using ROCK1 antibody (STJ29833) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts from wild type (WT) and ROCK1 knockout (KO) 293T cells, using ROCK1 antibody (STJ29833) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of C6, using ROCK1 antibody (STJ29833) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Western blot analysis of various lysates, using ROCK1 antibody (STJ29833) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-ROCK1 antibody (400-660) (STJ29833)

SKU:
STJ29833

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ROCK1 (400-660) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ROCK1
Gene ID: 6093
Uniprot ID: ROCK1_HUMAN
Immunogen Region: 400-660
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 400-660 of human ROCK1 (NP_005397.1).
Immunogen Sequence: YSNRRYLSSANPNDNRTSSN ADKSLQESLQKTIYKLEEQL HNEMQLKDEMEQKCRTSNIK LDKIMKELDEEGNQRRNLES TVSQIEKEKMLLQHRINEYQ RKAEQENEKRRNVENEVSTL KDQLEDLKKVSQNSQLANEK LSQLQKQLEEANDLLRTESD TAVRLRKSHTEMSKSISQLE SLNRELQERNRILENSKSQT DKDYYQLQAILEAERRDRGH DSEMIGDLQARITSLQEEV
Tissue Specificity Detected in blood platelets.
Post Translational Modifications Autophosphorylated on serine and threonine residues. Cleaved by caspase-3 during apoptosis. This leads to constitutive activation of the kinase and membrane blebbing.
Function Protein kinase which is a key regulator of the actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of DAPK3, GFAP, LIMK1, LIMK2, MYL9/MLC2, TPPP, PFN1 and PPP1R12A. Phosphorylates FHOD1 and acts synergistically with it to promote SRC-dependent non-apoptotic plasma membrane blebbing. Phosphorylates JIP3 and regulates the recruitment of JNK to JIP3 upon UVB-induced stress. Acts as a suppressor of inflammatory cell migration by regulating PTEN phosphorylation and stability. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Required for centrosome positioning and centrosome-dependent exit from mitosis. Plays a role in terminal erythroid differentiation. Inhibits podocyte motility via regulation of actin cytoskeletal dynamics and phosphorylation of CFL1. Promotes keratinocyte terminal differentiation. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. May regulate closure of the eyelids and ventral body wall by inducing the assembly of actomyosin bundles.
Protein Name Rho-Associated Protein Kinase 1
Renal Carcinoma Antigen Ny-Ren-35
Rho-Associated - Coiled-Coil-Containing Protein Kinase 1
Rho-Associated - Coiled-Coil-Containing Protein Kinase I
Rock-I
P160 Rock-1
P160rock
Database Links Reactome: R-HSA-111465
Reactome: R-HSA-3928662
Reactome: R-HSA-3928663
Reactome: R-HSA-416482
Reactome: R-HSA-416572
Reactome: R-HSA-4420097
Reactome: R-HSA-5627117
Reactome: R-HSA-6798695
Reactome: R-HSA-8980692
Reactome: R-HSA-9013026
Reactome: R-HSA-9013106
Reactome: R-HSA-9013407
Reactome: R-HSA-9013422
Reactome: R-HSA-9679191
Reactome: R-HSA-9696264
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Centriole
Golgi Apparatus Membrane
Peripheral Membrane Protein
Cell Projection
Bleb
Cell Membrane
Lamellipodium
Ruffle
A Small Proportion Is Associated With Golgi Membranes
Associated With The Mother Centriole And An Intercentriolar Linker
Colocalizes With Itgb1bp1 And Itgb1 At The Cell Membrane Predominantly In Lamellipodia And Membrane Ruffles
But Also In Retraction Fibers
Localizes At The Cell Membrane In An Itgb1bp1-Dependent Manner
Alternative Antibody Names Anti-Rho-Associated Protein Kinase 1 antibody
Anti-Renal Carcinoma Antigen Ny-Ren-35 antibody
Anti-Rho-Associated - Coiled-Coil-Containing Protein Kinase 1 antibody
Anti-Rho-Associated - Coiled-Coil-Containing Protein Kinase I antibody
Anti-Rock-I antibody
Anti-P160 Rock-1 antibody
Anti-P160rock antibody
Anti-ROCK1 antibody

Information sourced from Uniprot.org

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