![Western blot analysis of lysates from wild type (WT) and RBBP4 knockout (KO) 293T cells, using [KO Validated] RBBP4 Rabbit polyclonal antibody (STJ115869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/94876/376681/STJ115869_2__57285.1713143816.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and RBBP4 knockout (KO) 293T cells, using [KO Validated] RBBP4 Rabbit polyclonal antibody (STJ115869) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
Anti-RBBP4 antibody (1-425) (STJ115869)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RBBP4 (1-425) antibody for use in WB, ELISA and ChIP in human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA/ChIP |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements.ChIP:5 Mu g antibody for 10 Mu g-15 Mu g of Chromatin |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RBBP4 |
| Gene ID | 5928 |
| Uniprot ID | RBBP4_HUMAN |
| Immunogen Region | 1-425 |
| Immunogen Sequence | MADKEAAFDDAVEERVINEE YKIWKKNTPFLYDLVMTHAL EWPSLTAQWLPDVTRPEGKD FSIHRLVLGTHTSDEQNHLV IASVQLPNDDAQFDASHYDS EKGEFGGFGSVSGKIEIEIK INHEGEVNRARYMPQNPCII ATKTPSSDVLVFDYTKHPSK PDPSGECNPDLRLRGHQKEG YGLSWNPNLSGHLLSASDDH TICLWDISAVPKEGKVVDAK TIFTGHTAVVEDVSWHLLH |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-425 of human RBBP4 (NP_005601.1). |
Additional Info
| Tissue Specificity | Expressed in neuroblastoma cells. |
| Function | Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair. Component of the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression. Component of the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling. Component of the PRC2 complex, which promotes repression of homeotic genes during development. Component of the NURF (nucleosome remodeling factor) complex. |
| Protein Name | Histone-Binding Protein Rbbp4Chromatin Assembly Factor 1 Subunit CCaf-1 Subunit CChromatin Assembly Factor I P48 SubunitCaf-I 48 Kda SubunitCaf-I P48Nucleosome-Remodeling Factor Subunit Rbap48Retinoblastoma-Binding Protein 4Rbbp-4Retinoblastoma-Binding Protein P48 |
| Database Links | Reactome: R-HSA-1362277Reactome: R-HSA-1362300Reactome: R-HSA-1538133Reactome: R-HSA-156711Reactome: R-HSA-212300Reactome: R-HSA-2559580Reactome: R-HSA-3214815Reactome: R-HSA-3214841Reactome: R-HSA-427389Reactome: R-HSA-5617472Reactome: R-HSA-606279Reactome: R-HSA-6804758Reactome: R-HSA-69202Reactome: R-HSA-69205Reactome: R-HSA-69656Reactome: R-HSA-73762Reactome: R-HSA-8943724Reactome: R-HSA-8953750Reactome: R-HSA-9609690Reactome: R-HSA-9679191Reactome: R-HSA-9710421Reactome: R-HSA-9843940Reactome: R-HSA-9844594Reactome: R-HSA-9845323 |
| Cellular Localisation | NucleusChromosomeTelomereLocalizes To Chromatin As Part Of The Prc2 Complex |
| Alternative Antibody Names | Anti-Histone-Binding Protein Rbbp4 antibodyAnti-Chromatin Assembly Factor 1 Subunit C antibodyAnti-Caf-1 Subunit C antibodyAnti-Chromatin Assembly Factor I P48 Subunit antibodyAnti-Caf-I 48 Kda Subunit antibodyAnti-Caf-I P48 antibodyAnti-Nucleosome-Remodeling Factor Subunit Rbap48 antibodyAnti-Retinoblastoma-Binding Protein 4 antibodyAnti-Rbbp-4 antibodyAnti-Retinoblastoma-Binding Protein P48 antibodyAnti-RBBP4 antibodyAnti-RBAP48 antibody |
Information sourced from Uniprot.org