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Anti-RBBP4 antibody (1-100) [S4MR] (STJ11101914)

Western blot analysis of extracts from various cell lines, using RBBP4 rabbit monoclonal antibody (STJ11101914) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.
Immunohistochemistry analysis of paraffin-embedded rat brain using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded human lung cancer using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse brain using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded rat kidney using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded human placenta using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse liver using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded human breast cancer using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded human thyroid cancer using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded human tonsil using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded mouse intestin using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded human colon using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded human liver using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Immunohistochemistry analysis of paraffin-embedded human kidney using RBBP4 rabbit monoclonal antibody (STJ11101914) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
Confocal imaging of NIH/3T3 cells using RBBP4 rabbit monoclonal antibody (STJ11101914, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
Confocal imaging of U-2 OS cells using RBBP4 rabbit monoclonal antibody (STJ11101914, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-RBBP4 antibody (1-100) [S4MR] (STJ11101914)

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STJ11101914

£212.50 - £501.50

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit monoclonal antibody anti-RbAp48 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.

Clonality:	Monoclonal
Clone ID:	S4MR
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification:	Affinity purification
Dilution Range:	WB 1:500-1:1000 IHC-P 1:50-1:200 IF/ICC 1:50-1:200
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	RBBP4
Gene ID:	5928
Uniprot ID:	RBBP4_HUMAN
Immunogen Region:	1-100
Immunogen:	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human RBBP4 (NP_005601.1).
Immunogen Sequence:	MADKEAAFDDAVEERVINEE YKIWKKNTPFLYDLVMTHAL EWPSLTAQWLPDVTRPEGKD FSIHRLVLGTHTSDEQNHLV IASVQLPNDDAQFDASHYDS

Tissue Specificity	Expressed in neuroblastoma cells.
Function	Core histone-binding subunit that may target chromatin assembly factors, chromatin remodeling factors and histone deacetylases to their histone substrates in a manner that is regulated by nucleosomal DNA. Component of several complexes which regulate chromatin metabolism. These include the chromatin assembly factor 1 (CAF-1) complex, which is required for chromatin assembly following DNA replication and DNA repair.the core histone deacetylase (HDAC) complex, which promotes histone deacetylation and consequent transcriptional repression.the nucleosome remodeling and histone deacetylase complex (the NuRD complex), which promotes transcriptional repression by histone deacetylation and nucleosome remodeling.the PRC2 complex, which promotes repression of homeotic genes during development.and the NURF (nucleosome remodeling factor) complex.
Protein Name	Histone-Binding Protein Rbbp4 Chromatin Assembly Factor 1 Subunit C Caf-1 Subunit C Chromatin Assembly Factor I P48 Subunit Caf-I 48 Kda Subunit Caf-I P48 Nucleosome-Remodeling Factor Subunit Rbap48 Retinoblastoma-Binding Protein 4 Rbbp-4 Retinoblastoma-Binding Protein P48
Database Links	Reactome: R-HSA-1362277 Reactome: R-HSA-1362300 Reactome: R-HSA-1538133 Reactome: R-HSA-156711 Reactome: R-HSA-212300 Reactome: R-HSA-2559580 Reactome: R-HSA-3214815 Reactome: R-HSA-3214841 Reactome: R-HSA-427389 Reactome: R-HSA-5617472 Reactome: R-HSA-606279 Reactome: R-HSA-6804758 Reactome: R-HSA-69202 Reactome: R-HSA-69205 Reactome: R-HSA-69656 Reactome: R-HSA-73762 Reactome: R-HSA-8943724 Reactome: R-HSA-8953750 Reactome: R-HSA-9609690 Reactome: R-HSA-9679191 Reactome: R-HSA-9710421
Cellular Localisation	Nucleus Chromosome Telomere Localizes To Chromatin As Part Of The Prc2 Complex
Alternative Antibody Names	Anti-Histone-Binding Protein Rbbp4 antibody Anti-Chromatin Assembly Factor 1 Subunit C antibody Anti-Caf-1 Subunit C antibody Anti-Chromatin Assembly Factor I P48 Subunit antibody Anti-Caf-I 48 Kda Subunit antibody Anti-Caf-I P48 antibody Anti-Nucleosome-Remodeling Factor Subunit Rbap48 antibody Anti-Retinoblastoma-Binding Protein 4 antibody Anti-Rbbp-4 antibody Anti-Retinoblastoma-Binding Protein P48 antibody Anti-RBBP4 antibody Anti-RBAP48 antibody

Information sourced from Uniprot.org

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