• Western blot analysis of extracts of 293T cells, using [KO Validated] SETD7 antibody (STJ112026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts from normal (control) and SETD7 knockout (KO) 293T cells, using [KO Validated] SETD7 antibody (STJ112026) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-SETD7 antibody (1-366) (STJ112026)

SKU:
STJ112026

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-SETD7 (1-366) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: SETD7
Gene ID: 80854
Uniprot ID: SETD7_HUMAN
Immunogen Region: 1-366
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-366 of human SETD7 (NP_085151.1).
Immunogen Sequence: MDSDDEMVEEAVEGHLDDDG LPHGFCTVTYSSTDRFEGNF VHGEKNGRGKFFFFDGSTLE GYYVDDALQGQGVYTYEDGG VLQGTYVDGELNGPAQEYDT DGRLIFKGQYKDNIRHGVCW IYYPDGGSLVGEVNEDGEMT GEKIAYVYPDERTALYGKFI DGEMIEGKLATLMSTEEGRP HFELMPGNSVYHFDKSTSSC ISTNALLPDPYESERVYVAE SLISSAGEGLFSKVAVGPN
Tissue Specificity Widely expressed. Expressed in pancreatic islets.
Function Histone methyltransferase that specifically monomethylates 'Lys-4' of histone H3. H3 'Lys-4' methylation represents a specific tag for epigenetic transcriptional activation. Plays a central role in the transcriptional activation of genes such as collagenase or insulin. Recruited by IPF1/PDX-1 to the insulin promoter, leading to activate transcription. Has also methyltransferase activity toward non-histone proteins such as CGAS, p53/TP53, TAF10, and possibly TAF7 by recognizing and binding the KR-STA-K in substrate proteins. Monomethylates 'Lys-189' of TAF10, leading to increase the affinity of TAF10 for RNA polymerase II. Monomethylates 'Lys-372' of p53/TP53, stabilizing p53/TP53 and increasing p53/TP53-mediated transcriptional activation. Monomethylates 'Lys-491' of CGAS, promoting interaction between SGF29 and CGAS.
Protein Name Histone-Lysine N-Methyltransferase Setd7
Histone H3-K4 Methyltransferase Setd7
H3-K4-Hmtase Setd7
Lysine N-Methyltransferase 7
Set Domain-Containing Protein 7
Set7/9
Database Links Reactome: R-HSA-3214841
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone-Lysine N-Methyltransferase Setd7 antibody
Anti-Histone H3-K4 Methyltransferase Setd7 antibody
Anti-H3-K4-Hmtase Setd7 antibody
Anti-Lysine N-Methyltransferase 7 antibody
Anti-Set Domain-Containing Protein 7 antibody
Anti-Set7/9 antibody
Anti-SETD7 antibody
Anti-KIAA1717 antibody
Anti-KMT7 antibody
Anti-SET7 antibody
Anti-SET9 antibody

Information sourced from Uniprot.org

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