![Immunofluorescence analysis of L929 cells using [KO Validated] SEC61B Rabbit polyclonal antibody (STJ118233) at dilution of 1:100. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/97123/380111/STJ118233_1__50989.1713148036.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] SEC61B Rabbit polyclonal antibody (STJ118233) at dilution of 1:100. Blue: DAPI for nuclear staining.")
![Western blot analysis of lysates from wild type (WT) and SEC61B knockout (KO) 293T cells, using [KO Validated] SEC61B Rabbit polyclonal antibody (STJ118233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/97123/380112/STJ118233_2__03393.1713148037.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SEC61B knockout (KO) 293T cells, using [KO Validated] SEC61B Rabbit polyclonal antibody (STJ118233) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")
![Immunofluorescence analysis of L929 cells using [KO Validated] SEC61B Rabbit polyclonal antibody (STJ118233) at dilution of 1:100. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/97123/380111/STJ118233_1__50989.1713148036.jpg?c=1 "Immunofluorescence analysis of L929 cells using [KO Validated] SEC61B Rabbit polyclonal antibody (STJ118233) at dilution of 1:100. Blue: DAPI for nuclear staining.")
Anti-SEC61B antibody (1-96) (STJ118233)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SEC61B (1-96) antibody for use in WB, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.01% Thimerosal, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SEC61B |
| Gene ID | 10952 |
| Uniprot ID | SC61B_HUMAN |
| Immunogen Region | 1-96 |
| Immunogen Sequence | MPGPTPSGTNVGSSGRSPSK AVAARAAGSTVRQRKNASCG TRSAGRTTSAGTGGMWRFYT EDSPGLKVGPVPVLVMSLLF IASVFMLHIWGKYTRS |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 1-96 of human SEC61B (NP_006799.1). |
Additional Info
| Function | Component of SEC61 channel-forming translocon complex that mediates transport of signal peptide-containing precursor polypeptides across the endoplasmic reticulum (ER). Forms a ribosome receptor and a gated pore in the ER membrane, both functions required for cotranslational translocation of nascent polypeptides. The SEC61 channel is also involved in ER membrane insertion of transmembrane proteins: it mediates membrane insertion of the first few transmembrane segments of proteins, while insertion of subsequent transmembrane regions of multi-pass membrane proteins is mediated by the multi-pass translocon (MPT) complex. The SEC61 channel cooperates with the translocating protein TRAM1 to import nascent proteins into the ER. |
| Protein Name | Protein Transport Protein Sec61 Subunit Beta |
| Database Links | Reactome: R-HSA-1236974Reactome: R-HSA-1799339Reactome: R-HSA-9609523 |
| Cellular Localisation | Endoplasmic Reticulum MembraneSingle-Pass Membrane Protein |
| Alternative Antibody Names | Anti-Protein Transport Protein Sec61 Subunit Beta antibodyAnti-SEC61B antibody |
Information sourced from Uniprot.org