• Western blot analysis of lysates from wild type (WT) and RAB2A knockout (KO) 293T cells, using [KO Validated] RAB2A Rabbit polyclonal antibody (STJ11100858) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-RAB2A antibody (1-212) (STJ11100858)

SKU:
STJ11100858

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-RAB2A (1-212) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: RAB2A
Gene ID: 5862
Uniprot ID: RAB2A_HUMAN
Immunogen Region: 1-212
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human RAB2A (NP_002856.1).
Immunogen Sequence: MAYAYLFKYIIIGDTGVGKS CLLLQFTDKRFQPVHDLTIG VEFGARMITIDGKQIKLQIW DTAGQESFRSITRSYYRGAA GALLVYDITRRDTFNHLTTW LEDARQHSNSNMVIMLIGNK SDLESRREVKKEEGEAFARE HGLIFMETSAKTASNVEEAF INTAKEIYEKIQEGVFDINN EANGIKIGPQHAATNATHAG NQGGQQAGGGCC
Function The small GTPases Rab are key regulators of intracellular membrane trafficking, from the formation of transport vesicles to their fusion with membranes. Rabs cycle between active GTP-bound and inactive GDP-bound states. In their active state, drive transport of vesicular carriers from donor organelles to acceptor organelles to regulate the membrane traffic that maintains organelle identity and morphology. Required for protein transport from the endoplasmic reticulum to the Golgi complex. Regulates the compacted morphology of the Golgi.
Protein Name Ras-Related Protein Rab-2a
Database Links Reactome: R-HSA-162658
Reactome: R-HSA-8873719
Cellular Localisation Endoplasmic Reticulum-Golgi Intermediate Compartment Membrane
Lipid-Anchor
Melanosome
Endoplasmic Reticulum Membrane
Golgi Apparatus Membrane
Cytoplasmic Vesicle
Secretory Vesicle
Acrosome
Localized In The Golgi Apparatus In The Round Spermatids And In The Acrosome In The Elongating Spermatid
Identified By Mass Spectrometry In Melanosome Fractions From Stage I To Stage Iv
Alternative Antibody Names Anti-Ras-Related Protein Rab-2a antibody
Anti-RAB2A antibody
Anti-RAB2 antibody

Information sourced from Uniprot.org

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