![Western blot analysis of various lysates using [KO Validated] SHMT2 Rabbit polyclonal antibody (STJ25526) at 1:400 dilution incubated overnight at 4 ยฐC. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic KitExposure time: 30 s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/100514/387105/STJ25526_1__79760.1713156306.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] SHMT2 Rabbit polyclonal antibody (STJ25526) at 1:400 dilution incubated overnight at 4 ยฐC. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic KitExposure time: 30 s.")
![Western blot analysis of lysates from wild type (WT) and SHMT2 knockout (KO) HeLa cells using [KO Validated] SHMT2 Rabbit polyclonal antibody (STJ25526) at 1:400 dilution incubated overnight at 4 ยฐC. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic KitExposure time: 30 s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/100514/387106/STJ25526_2__64615.1713156307.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and SHMT2 knockout (KO) HeLa cells using [KO Validated] SHMT2 Rabbit polyclonal antibody (STJ25526) at 1:400 dilution incubated overnight at 4 ยฐC. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic KitExposure time: 30 s.")
![Western blot analysis of various lysates using [KO Validated] SHMT2 Rabbit polyclonal antibody (STJ25526) at 1:400 dilution incubated overnight at 4 ยฐC. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic KitExposure time: 30 s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/100514/387105/STJ25526_1__79760.1713156306.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] SHMT2 Rabbit polyclonal antibody (STJ25526) at 1:400 dilution incubated overnight at 4 ยฐC. Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic KitExposure time: 30 s.")
Anti-SHMT2 antibody (265-504) (STJ25526)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal SHMT2 (265-504) antibody for use in WB, IHC-P and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | SHMT2 |
| Gene ID | 6472 |
| Uniprot ID | GLYM_HUMAN |
| Immunogen Region | 265-504 |
| Immunogen Sequence | PSPFKHADIVTTTTHKTLRG ARSGLIFYRKGVKAVDPKTG REIPYTFEDRINFAVFPSLQ GGPHNHAIAAVAVALKQACT PMFREYSLQVLKNARAMADA LLERGYSLVSGGTDNHLVLV DLRPKGLDGARAERVLELVS ITANKNTCPGDRSAITPGGL RLGAPALTSRQFREDDFRRV VDFIDEGVNIGLEVKSKTAK LQDFKSFLLKDSETSQRLAN LRQRVEQFARAFPMPGFDE |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 265-504 of human SHMT2 (NP_005403.2). |
Additional Info
| Post Translational Modifications | Succinylation at Lys-280 inhibits the hydroxymethyltransferase activity. Desuccinylation by SIRT5 restores the activity, leading to promote cell proliferation. |
| Function | Catalyzes the cleavage of serine to glycine accompanied with the production of 5,10-methylenetetrahydrofolate, an essential intermediate for purine biosynthesis. Serine provides the major source of folate one-carbon in cells by catalyzing the transfer of one carbon from serine to tetrahydrofolate. Contributes to the de novo mitochondrial thymidylate biosynthesis pathway via its role in glycine and tetrahydrofolate metabolism: thymidylate biosynthesis is required to prevent uracil accumulation in mtDNA. Also required for mitochondrial translation by producing 5,10-methylenetetrahydrofolate.5,10-methylenetetrahydrofolate providing methyl donors to produce the taurinomethyluridine base at the wobble position of some mitochondrial tRNAs. Associates with mitochondrial DNA. In addition to its role in mitochondria, also plays a role in the deubiquitination of target proteins as component of the BRISC complex: required for IFNAR1 deubiquitination by the BRISC complex. |
| Protein Name | Serine Hydroxymethyltransferase - MitochondrialShmtGlycine HydroxymethyltransferaseSerine Methylase |
| Database Links | Reactome: R-HSA-196757Reactome: R-HSA-9013408Reactome: R-HSA-9837999 |
| Cellular Localisation | MitochondrionMitochondrion MatrixMitochondrion NucleoidMitochondrion Inner MembraneCytoplasmNucleusMainly Localizes In The MitochondrionAlso Found In The Cytoplasm And Nucleus As Part Of The Brisc Complex |
| Alternative Antibody Names | Anti-Serine Hydroxymethyltransferase - Mitochondrial antibodyAnti-Shmt antibodyAnti-Glycine Hydroxymethyltransferase antibodyAnti-Serine Methylase antibodyAnti-SHMT2 antibody |
Information sourced from Uniprot.org