| Host: |
Rabbit |
| Applications: |
WB |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-SETDB1 (1-397) is suitable for use in Western Blot research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:500-1:1000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
SETDB1 |
| Gene ID: |
9869 |
| Uniprot ID: |
SETB1_HUMAN |
| Immunogen Region: |
1-397 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-397 of human SETDB1 (NP_001230420.1). |
| Immunogen Sequence: |
MSSLPGCIGLDAATATVESE EIAELQQAVVEELGISMEEL RHFIDEELEKMDCVQQRKKQ LAELETWVIQKESEVAHVDQ LFDDASRAVTNCESLVKDFY SKLGLQYRDSSSEDESSRPT EIIEIPDEDDDVLSIDSGDA GSRTPKDQKLREAMAALRKS AQDVQKFMDAVNKKSSSQDL HKGTLSQMSGELSKDGDLIV SMRILGKKRTKTWHKGTLIA IQTVGPGKKYKVKFDNKGK |
| Tissue Specificity | Widely expressed. High expression in testis. |
| Post Translational Modifications | Degraded by the proteasome, shielded by interaction with ATF7IP. |
| Function | Histone methyltransferase that specifically trimethylates 'Lys-9' of histone H3. H3 'Lys-9' trimethylation represents a specific tag for epigenetic transcriptional repression by recruiting HP1 (CBX1, CBX3 and/or CBX5) proteins to methylated histones. Mainly functions in euchromatin regions, thereby playing a central role in the silencing of euchromatic genes. H3 'Lys-9' trimethylation is coordinated with DNA methylation. Required for HUSH-mediated heterochromatin formation and gene silencing. Forms a complex with MBD1 and ATF7IP that represses transcription and couples DNA methylation and histone 'Lys-9' trimethylation. Its activity is dependent on MBD1 and is heritably maintained through DNA replication by being recruited by CAF-1. SETDB1 is targeted to histone H3 by TRIM28/TIF1B, a factor recruited by KRAB zinc-finger proteins. Probably forms a corepressor complex required for activated KRAS-mediated promoter hypermethylation and transcriptional silencing of tumor suppressor genes (TSGs) or other tumor-related genes in colorectal cancer (CRC) cells. Required to maintain a transcriptionally repressive state of genes in undifferentiated embryonic stem cells (ESCs). In ESCs, in collaboration with TRIM28, is also required for H3K9me3 and silencing of endogenous and introduced retroviruses in a DNA-methylation independent-pathway. Associates at promoter regions of tumor suppressor genes (TSGs) leading to their gene silencing. The SETDB1-TRIM28-ZNF274 complex may play a role in recruiting ATRX to the 3'-exons of zinc-finger coding genes with atypical chromatin signatures to establish or maintain/protect H3K9me3 at these transcriptionally active regions. |
| Protein Name | Histone-Lysine N-Methyltransferase Setdb1Erg-Associated Protein With Set DomainEsetHistone H3-K9 Methyltransferase 4H3-K9-Hmtase 4Lysine N-Methyltransferase 1eSet Domain Bifurcated 1 |
| Database Links | Reactome: R-HSA-3214841 |
| Cellular Localisation | NucleusCytoplasmChromosomeAssociated With Non-Pericentromeric Regions Of ChromatinExcluded From Nucleoli And Islands Of Condensed Chromatin |
| Alternative Antibody Names | Anti-Histone-Lysine N-Methyltransferase Setdb1 antibodyAnti-Erg-Associated Protein With Set Domain antibodyAnti-Eset antibodyAnti-Histone H3-K9 Methyltransferase 4 antibodyAnti-H3-K9-Hmtase 4 antibodyAnti-Lysine N-Methyltransferase 1e antibodyAnti-Set Domain Bifurcated 1 antibodyAnti-SETDB1 antibodyAnti-ESET antibodyAnti-KIAA0067 antibodyAnti-KMT1E antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] DNAJA1 Rabbit polyclonal antibody (STJ113207) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/92785/373370/STJ113207_1__86638.1713139598.jpg?c=1 "Immunofluorescence analysis of NIH/3T3 cells using [KO Validated] DNAJA1 Rabbit polyclonal antibody (STJ113207) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")