Western Blot Antibodies | St John's Laboratory

Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g Alpha-Actinin-4 antibody (STJ11101857). Western blot was performed from the immunoprecipitate using Alpha-Actinin-4 antibody (STJ11101857) at a dilution of 1:1000.

Anti-ACTN4 antibody (1-100) [S7MR] (STJ11101857)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunocytochemistry analysis of alpha Actinin 4 (green) in Hela using alpha Actinin 4 antibody, and DAPI (blue)

Anti-ACTN4 antibody (1-14 aa) [R06-3D7] (STJA0010939)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Mouse/Rat

$332.78

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Western blot with Anti-ACTN4 Antibody (STJ500045) of 1) rat podocyte membranes and 2) NIH3TS3 cells. Antibody was diluted 1:500 in buffer.

Anti-ACTN4 antibody (140-190) (STJ500045)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$629.78

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Anti-ACTN4 antibody (140-190) {Biotin} (STJ500046)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$713.48

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Anti-ACTN4 antibody (140-190) {FITC} (STJ500047)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

$713.48

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Western blot analysis of various lysates using Alpha-Actinin-4 Rabbit polyclonal antibody (STJ118740) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ACTN4 antibody (570-650) (STJ118740)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-ACTN4 antibody (N-Term) (STJA0005245)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$431.33

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Anti-ACTN4 antibody (STJA0006842)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using ACTR10 Rabbit polyclonal antibody (STJ118312) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ACTR10 antibody (218-417) (STJ118312)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-ACTR10 antibody (STJA0007293)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using ACTR1A antibody (STJ28598) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-ACTR1A antibody (187-376) (STJ28598)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-ACTR1A antibody (260-310) (STJA0006448)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of ACTR1B in paraffin-embedded Human colon carcinoma using ACTR1B Rabbit polyclonal antibody (STJ118218) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ACTR1B antibody (1-105) (STJ118218)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from AD293 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ACTR1B antibody (29-79 aa) (STJ195888)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-ACTR1B antibody [PT0778R] (STJA0039339)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ICC/FC/IP

Reactivity :

Human

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Immunofluorescence analysis of U2OS cells using Arp2 antibody (STJ28301). Blue: DAPI for nuclear staining.

Anti-ACTR2 antibody (1-160) (STJ28301)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunocytochemistry analysis of Actin Related Protein 2 (green) in Hela using Actin Related Protein 2 antibody, and DAPI (blue).

Anti-ACTR2 antibody (374-387 aa) [R04-3H3] (STJA0012273)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse/Rat

$357.08

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Anti-ACTR2 antibody (C-Term) (STJA0005266)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IP

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$493.43

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Anti-ACTR2 antibody (STJA0006991)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of HeLa cells using Arp3 antibody (STJ22503). Blue: DAPI for nuclear staining.

Anti-ACTR3 antibody (1-418) (STJ22503)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Confocal imaging of U-2 OS cells using Arp3 Rabbit monoclonal antibody (STJ11102091, dilution 1:100) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody (dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Anti-ACTR3 antibody (319-418) [S1MR] (STJ11102091)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Western blot analysis of various cells using Arp3 Polyclonal Antibody

Anti-ACTR3 antibody (369-418 aa) (STJ91701)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunocytochemistry analysis of Arp3 (green) in Hela cells using Arp3 antibody, and DAPI (blue)

Anti-ACTR3 antibody (394-407 aa) [R08-2A2] (STJA0010983)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Mouse/Rat

$332.78

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Anti-ACTR3 antibody (C-Term) (STJA0005268)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC/IHC

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$431.33

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Western blot analysis of extracts of various cell lines, using ACTR3B antibody (STJ110134) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ACTR3B antibody (189-418) (STJ110134)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Anti-ACTR3B antibody (STJA0009635)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Anti-ACTR3C antibody (86-136 aa) (STJ197912)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-ACTR5 antibody (STJA0007378)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of lysates from 293T cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-ACTRT1 antibody (241-291 aa) (STJ194507)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-ACTRT2 antibody (STJA0009306)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of various lysates using ACTRT3 Rabbit polyclonal antibody (STJ115164) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ACTRT3 antibody (1-130) (STJ115164)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-ACTRT3 antibody (STJA0008311)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using ACVR1 antibody (STJ29680) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-ACVR1 antibody (20-125) (STJ29680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of extracts of mouse brain, using ACVR1 antibody (STJ11103005) at1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ACVR1 antibody (410-509) [S5MR] (STJ11103005)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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STJ71385 (0. 3µg/ml) staining of Human Umbilical Cord lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-ACVR1 antibody (Internal) (STJ71385)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human/Mouse/Rat/Dog

$421.88

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Biotinylated STJ71385 (0. 1µg/ml) staining of Human Umbilical Cord lysate (35µg protein in RIPA buffer) , exactly mirroring its parental non-biotinylated product. Primary incubation was 1 hour. Detected by chemiluminescence, using streptavidin-HRP and using NAP blocker as a substitute for skimmed milk.

Anti-ACVR1, Biotinylated antibody (Internal) {Biotin} (STJ73238)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Mouse/Rat/Dog

$421.88

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Western blot analysis of extracts of various cell lines, using ACVR1B rabbit monoclonal antibody (STJ11101804) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ACVR1B antibody (1-100) [S4MR] (STJ11101804)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-ACVR1B antibody (24-126) (STJ119153)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using ACVR1B antibody (STJ27406) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ACVR1B antibody (24-126) (STJ27406)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Immunocytochemistry analysis of Activin A Receptor Type IB (green) in hela using Activin A Receptor Type IB antibody, and DAPI (blue)

Anti-ACVR1B antibody (63-77 aa) [R08-8D6] (STJA0010911)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Mouse/Rat/Hamster

$332.78

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Western blot analysis of various cells using ACTR-IB Polyclonal Antibody diluted at 1：1000

Anti-ACVR1B antibody (73-122 aa) (STJ91471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunohistochemistry analysis of ACVR1C in paraffin-embedded mouse stomach using ACVR1C Rabbit polyclonal antibody (STJ22505) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-ACVR1C antibody (22-113) (STJ22505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using ACVR2A rabbit monoclonal antibody (STJ11102305) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-ACVR2A antibody (1-100) [S5MR] (STJ11102305)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Western blot analysis of lysates from rat kidney, using ACTR-IIA antibody.

Anti-ACVR2A antibody (10-59 aa) (STJ91473)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Immunofluorescence analysis of NIH/3T3 cells using ACVR2A Rabbit polyclonal antibody (STJ22506) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-ACVR2A antibody (20-513) (STJ22506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of Activin Receptor Type IIB/ACVR2B in CHO-K1, mouse Brain lysates using Activin Receptor Type IIB antibody.

Anti-ACVR2B antibody (1-100 aa) [R01-2I1] (STJA0012257)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat/Hamster

$357.08

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Anti-ACVR2B antibody (40-120 aa) (STJ190499)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of various lysates using ACVR2B Rabbit pAb (STJ117842) at 1:3000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-ACVR2B antibody (50-150) (STJ117842)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$151.88 - $708.08

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