• Western blot analysis of extracts of various cell lines, using ACSS2 antibody (STJ115557) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-ACSS2 antibody (462-701) (STJ115557)

SKU:
STJ115557

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Host: Rabbit
Applications: WB
Reactivity: Human/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACSS2 (462-701) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACSS2
Gene ID: 55902
Uniprot ID: ACSA_HUMAN
Immunogen Region: 462-701
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 462-701 of human ACSS2 (NP_061147.1).
Immunogen Sequence: VDTFWQTETGGHMLTPLPGA TPMKPGSATFPFFGVAPAIL NESGEELEGEAEGYLVFKQP WPGIMRTVYGNHERFETTYF KKFPGYYVTGDGCQRDQDGY YWITGRIDDMLNVSGHLLST AEVESALVEHEAVAEAAVVG HPHPVKGECLYCFVTLCDGH TFSPKLTEELKKQIREKIGP IATPDYIQNAPGLPKTRSGK IMRRVLRKIAQNDHDLGDMS TVADPSVISHLFSHRCLTI
Post Translational Modifications Reversibly acetylated at Lys-661. The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylases SIRT1 and SIRT3.
Function Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity.
Protein Name Acetyl-Coenzyme A Synthetase - Cytoplasmic
Acetate--Coa Ligase
Acetyl-Coa Synthetase
Acs
Acecs
Acetyl-Coa Synthetase 1
Acecs1
Acyl-Coa Synthetase Short-Chain Family Member 2
Acyl-Activating Enzyme
Propionate--Coa Ligase
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-71384
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Acetyl-Coenzyme A Synthetase - Cytoplasmic antibody
Anti-Acetate--Coa Ligase antibody
Anti-Acetyl-Coa Synthetase antibody
Anti-Acs antibody
Anti-Acecs antibody
Anti-Acetyl-Coa Synthetase 1 antibody
Anti-Acecs1 antibody
Anti-Acyl-Coa Synthetase Short-Chain Family Member 2 antibody
Anti-Acyl-Activating Enzyme antibody
Anti-Propionate--Coa Ligase antibody
Anti-ACSS2 antibody
Anti-ACAS2 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance