• Western blot analysis of extracts of various cell lines, using ACSS2 rabbit monoclonal antibody (STJ11101408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.
  • Western blot analysis of extracts of HepG2 cells, using ACSS2 rabbit monoclonal antibody (STJ11101408) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.

Anti-ACSS2 antibody (50-150) [S8MR] (STJ11101408)

SKU:
STJ11101408

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Host: Rabbit
Applications: WB
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-ACSS2 (50-150) is suitable for use in Western Blot research applications.
Clonality: Monoclonal
Clone ID: S8MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACSS2
Gene ID: 55902
Uniprot ID: ACSA_HUMAN
Immunogen Region: 50-150
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 50-150 of human ACSS2 (Q9NR19).
Immunogen Sequence: LHRRSVEEPREFWGDIAKEF YWKTPCPGPFLRYNFDVTKG KIFIEWMKGATTNICYNVLD RNVHEKKLGDKVAFYWEGNE PGETTQITYHQLLVQVCQFS N
Post Translational Modifications Reversibly acetylated at Lys-661. The acetyl-CoA synthase activity is inhibited by acetylation and activated by deacetylation mediated by the deacetylases SIRT1 and SIRT3.
Function Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Acetate is the preferred substrate. Can also utilize propionate with a much lower affinity.
Protein Name Acetyl-Coenzyme A Synthetase - Cytoplasmic
Acetate--Coa Ligase
Acetyl-Coa Synthetase
Acs
Acecs
Acetyl-Coa Synthetase 1
Acecs1
Acyl-Coa Synthetase Short-Chain Family Member 2
Acyl-Activating Enzyme
Propionate--Coa Ligase
Database Links Reactome: R-HSA-2151201
Reactome: R-HSA-71384
Cellular Localisation Cytoplasm
Cytosol
Alternative Antibody Names Anti-Acetyl-Coenzyme A Synthetase - Cytoplasmic antibody
Anti-Acetate--Coa Ligase antibody
Anti-Acetyl-Coa Synthetase antibody
Anti-Acs antibody
Anti-Acecs antibody
Anti-Acetyl-Coa Synthetase 1 antibody
Anti-Acecs1 antibody
Anti-Acyl-Coa Synthetase Short-Chain Family Member 2 antibody
Anti-Acyl-Activating Enzyme antibody
Anti-Propionate--Coa Ligase antibody
Anti-ACSS2 antibody
Anti-ACAS2 antibody

Information sourced from Uniprot.org

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