• Western blot analysis of various lysates using ACSS3 Rabbit polyclonal antibody (STJ115726) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-ACSS3 antibody (30-280) (STJ115726)

SKU:
STJ115726

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Host: Rabbit
Applications: WB
Reactivity: Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ACSS3 (30-280) is suitable for use in Western Blot research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACSS3
Gene ID: 79611
Uniprot ID: ACSS3_HUMAN
Immunogen Region: 30-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 30-280 of human ACSS3 (NP_078836.1).
Immunogen Sequence: AGAALRALVVPGPRGGLGGR GCRALSSGSGSEYKTHFAAS VTDPERFWGKAAEQISWYKP WTKTLENKHSPSTRWFVEGM LNICYNAVDRHIENGKGDKI AIIYDSPVTNTKATFTYKEV LEQVSKLAGVLVKHGIKKGD TVVIYMPMIPQAMYTMLACA RIGAIHSLIFGGFASKELSS RIDHVKPKVVVTASFGIEPG RRVEYVPLVEEALKIGQHKP DKILIYNRPNMEAVPLAPG
Function Catalyzes the synthesis of acetyl-CoA from short-chain fatty acids. Propionate is the preferred substrate. Can utilize acetate and butyrate with a much lower affinity.
Protein Name Acyl-Coa Synthetase Short-Chain Family Member 3 - Mitochondrial
Acetate--Coa Ligase 3
Acyl-Coa Synthetase Short-Chain Family Member 3
Propionate--Coa Ligase
Database Links Reactome: R-HSA-77111
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Acyl-Coa Synthetase Short-Chain Family Member 3 - Mitochondrial antibody
Anti-Acetate--Coa Ligase 3 antibody
Anti-Acyl-Coa Synthetase Short-Chain Family Member 3 antibody
Anti-Propionate--Coa Ligase antibody
Anti-ACSS3 antibody

Information sourced from Uniprot.org

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