• Western blot analysis of extracts of various cell lines, using Alpha-Smooth Muscle Actin (ACTA2) antibody (STJ114915) at 1:500 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Immunohistochemistry of paraffin-embedded human placenta using Alpha-Smooth Muscle Actin (ACTA2) antibody (STJ114915) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded rat ovary using Alpha-Smooth Muscle Actin (ACTA2) antibody (STJ114915) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry of paraffin-embedded mouse lung using Alpha-Smooth Muscle Actin (ACTA2) antibody (STJ114915) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of rat rectum using Alpha-Smooth Muscle Actin (ACTA2) rabbit polyclonal antibody (STJ114915) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of human smooth muscle using Alpha-Smooth Muscle Actin (ACTA2) rabbit polyclonal antibody (STJ114915) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of mouse colon using Alpha-Smooth Muscle Actin (ACTA2) rabbit polyclonal antibody (STJ114915) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ACTA2 antibody (1-377) (STJ114915)

SKU:
STJ114915

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-alpha smooth muscle actin (1-377) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ACTA2
Gene ID: 59
Uniprot ID: ACTA_HUMAN
Immunogen Region: 1-377
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-377 of human Alpha-Smooth Muscle Actin (ACTA2) (NP_001604.1).
Immunogen Sequence: MCEEEDSTALVCDNGSGLCK AGFAGDDAPRAVFPSIVGRP RHQGVMVGMGQKDSYVGDEA QSKRGILTLKYPIEHGIITN WDDMEKIWHHSFYNELRVAP
Post Translational Modifications Oxidation of Met-46 and Met-49 by MICALs (MICAL1, MICAL2 or MICAL3) to form methionine sulfoxide promotes actin filament depolymerization. MICAL1 and MICAL2 produce the (R)-S-oxide form. The (R)-S-oxide form is reverted by MSRB1 and MSRB2, which promotes actin repolymerization. Monomethylation at Lys-86 (K84me1) regulates actin-myosin interaction and actomyosin-dependent processes. Demethylation by ALKBH4 is required for maintaining actomyosin dynamics supporting normal cleavage furrow ingression during cytokinesis and cell migration. Methylated at His-75 by SETD3. Actin, aortic smooth muscle, intermediate form: N-terminal cleavage of acetylated cysteine of intermediate muscle actin by ACTMAP. (Microbial infection) Monomeric actin is cross-linked by V.cholerae toxins RtxA and VgrG1 in case of infection: bacterial toxins mediate the cross-link between Lys-52 of one monomer and Glu-272 of another actin monomer, resulting in formation of highly toxic actin oligomers that cause cell rounding. The toxin can be highly efficient at very low concentrations by acting on formin homology family proteins: toxic actin oligomers bind with high affinity to formins and adversely affect both nucleation and elongation abilities of formins, causing their potent inhibition in both profilin-dependent and independent manners.
Function Actins are highly conserved proteins that are involved in various types of cell motility and are ubiquitously expressed in all eukaryotic cells.
Protein Name Actin - Aortic Smooth Muscle
Alpha-Actin-2
Cell Growth-Inhibiting Gene 46 Protein Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form
Database Links Reactome: R-HSA-445355
Reactome: R-HSA-9013695
Cellular Localisation Cytoplasm
Cytoskeleton
Alternative Antibody Names Anti-Actin - Aortic Smooth Muscle antibody
Anti-Alpha-Actin-2 antibody
Anti-Cell Growth-Inhibiting Gene 46 Protein Cleaved Into - Actin - Aortic Smooth Muscle - Intermediate Form antibody
Anti-ACTA2 antibody
Anti-ACTSA antibody
Anti-ACTVS antibody
Anti-GIG46 antibody

Information sourced from Uniprot.org

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