Immunocytochemistry Antibodies | St John's Laboratory

Anti-SLC5A1 antibody (603-623) {Biotin} (STJ502989)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat/Sheep

$713.48

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Anti-SLC5A1 antibody (603-623) {FITC} (STJ502990)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat/Sheep

$713.48

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Western blot analysis of various lysates, using SLC5A2 rabbit polyclonal antibody (STJ11103251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.

Anti-SLC5A2 antibody (564-624) (STJ11103251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry of mouse kidney with Anti-SLC5A2 primary antibody (STJ502993) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain.

Anti-SLC5A2 antibody (580-630) (STJ502993)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse

$577.13

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Anti-SLC5A2 antibody (580-630) {Biotin} (STJ502994)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse

$713.48

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Anti-SLC5A2 antibody (580-630) {FITC} (STJ502995)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse

$713.48

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Western blot analysis of various lysates using SLC5A7 Rabbit pAb (STJ11100952) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-SLC5A7 antibody (503-580) (STJ11100952)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry of rat brain with Anti-SLC6A2 primary antibody (STJ501915) at 1:100 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SLC6A2 antibody (N-Term) (STJ501915)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$629.78

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Anti-SLC6A2 antibody (N-Term) {Biotin} (STJ501916)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-SLC6A2 antibody (N-Term) {FITC} (STJ501917)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Immunofluorescence analysis of mouse brain using Dopamine Transporter/SLC6A3 Rabbit polyclonal antibody (STJ117430) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-SLC6A3 antibody (1-100) (STJ117430)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry of mouse brain with Anti-SLC7A10 primary antibody (STJ500165) at 1:50 dilution in Buffer. Section was treated with DAB and Haematoxylin stain. Magnification is at 40X.

Anti-SLC7A10 antibody (1-50) (STJ500165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Mouse/Rat

$629.78

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Anti-SLC7A10 antibody (1-50) {Biotin} (STJ500169)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Mouse/Rat

$713.48

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Anti-SLC7A10 antibody (1-50) {FITC} (STJ500170)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Mouse/Rat

$713.48

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Western blot with Anti-SLC7A10 Antibody (STJ500166) of mouse brain at 1:250 dilution in buffer, 1% milk overnight .

Anti-SLC7A10 antibody (450-500) (STJ500166)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$629.78

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Anti-SLC7A10 antibody (450-500) {FITC} (STJ500168)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-SLC7A10 antibody (C-Term) {Biotin} (STJ500167)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

CM/ELISA/ICC/IF/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Immunofluorescence analysis of MCF7 cells using LAT1/SLC7A5 Rabbit polyclonal antibody (STJ25593) at dilution of 1:300 (40x lens). Blue: DAPI for nuclear staining.

Anti-SLC7A5 antibody (1-125) (STJ25593)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-SLC7A9 antibody (1-100) (STJ114714)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunofluorescence analysis of NIH/3T3 cells using Smad1 Rabbit polyclonal antibody (STJ11104508) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Anti-SMAD1 antibody (20-240) (STJ11104508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from C2C12 cells using Smad1/Smad5/Smad9 Rabbit pAb (STJ11106932) at1:1000 dilution. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>Exposure time: 15s.

Anti-Smad1/Smad5/Smad9 antibody (19-125 aa) (STJ11106932)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

$151.88 - $708.08

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Western blot analysis of extracts from normal (control) and Smad2 knockout (KO) HeLa cells, using Smad2 antibody (STJ110012) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5min.

Anti-SMAD2 antibody (1-100) (STJ110012)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g Smad2 antibody (STJ11101651). Western blot was performed from the immunoprecipitate using Smad2 antibody (STJ11101651) at a dilution of 1:1000.

Anti-SMAD2 antibody (1-100) [S1MR] (STJ11101651)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunocytochemistry analysis of Smad2 (green) in Hela using Smad2 antibody, and DAPI (blue).

Anti-SMAD2 antibody (81-100 aa) [R03-3F8] (STJA0013074)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF/IP

Reactivity :

Human/Rat/Hamster

$357.08

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Western blot detection of Smad2 in Hela, A431, Jurkat and K562 cell lysates using Smad2 mouse mAb (1:500 diluted).Predicted band size:60KDa.Observed band size:60KDa.

Anti-SMAD2 antibody [6H5-E3-C11] (STJ99174)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC

Reactivity :

Human

$194.40 - $469.80

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Western blot analysis of various lysates using SMAD2/SMAD3 Rabbit pAb (STJ11107460) at 1:700 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-SMAD2/SMAD3 antibody (150-250 aa) (STJ11107460)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of various lysates using SMAD2/SMAD3 Rabbit pAb (STJ11101471) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-SMAD2/SMAD3 antibody (150-270) (STJ11101471)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of lysates from wild type (WT) and 293T cells transfected with SMAD2/SMAD3 using SMAD2/SMAD3 Rabbit pAb (STJ11106981) at1:1000 dilution incubated overnight at 4°C. <br>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br>Lysates/proteins: 25 Mu g per lane. <br>Blocking buffer: 3% nonfat dry milk in TBST. <br>Detection: ECL Basic Kit <br>NC:293T cells transfected with SMAD5<br>Exposure time: 10s.

Anti-SMAD2/SMAD3 antibody (175-270 aa) (STJ11106981)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ChIP

Reactivity :

Human

$151.88 - $708.08

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Immunofluorescence analysis of NIH/3T3 cells using Smad3 Rabbit polyclonal antibody (STJ11101192) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SMAD3 antibody (150-425) (STJ11101192)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunocytochemistry analysis of Smad3 (green) in A549 using Smad3 antibody, and DAPI (blue).

Anti-SMAD3 antibody (202-219 aa) [R09-7H4] (STJA0010795)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

$332.78

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Immunocytochemistry analysis of Smad4 (green) in Hela using Smad4 antibody, and DAPI (blue).

Anti-SMAD4 antibody (316-552 aa) [R05-1I7] (STJA0012592)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

$357.08

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Western blot analysis of lysates from HCT116 cells, using Smad4 Rabbit pAb (STJ27624) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-SMAD4 antibody (320-552) (STJ27624)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunohistochemistry analysis of paraffin-embedded Human lung cancer using SMAD5 antibody. High-pressure and temperature Sodium Citrate pH 6. 0 was used for antigen retrieval.

Anti-SMAD5 antibody (244-262 aa) [R07-2A5] (STJA0011975)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human/Rat

$357.08

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Western blot detection of SMAD5 (C-terminus) in Hela, Jurkat and K562 cell lysates using SMAD5 (C-terminus) mouse mAb (1:1000 diluted).Predicted band size:52KDa.Observed band size:60KDa.

Anti-SMAD5 antibody (C-Term) [4B10-B10-B6] (STJ99081)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/FC

Reactivity :

Human

$194.40 - $469.80

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Immunofluorescence analysis of NIH-3T3 cells using Smad6 antibody (STJ116949) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-SMAD6 antibody (257-496) (STJ116949)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using Smad7 antibody (STJ114223) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-SMAD7 antibody (220-420) (STJ114223)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-SMAD7 antibody (N-Term) (STJA0005586)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Human/Mouse/Rat/Xenopus

$431.33

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Immunofluorescence analysis of L929 cells using SMAP1 Rabbit polyclonal antibody (STJ119790) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-SMAP1 antibody (180-250) (STJ119790)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Western blot analysis of extracts of various cell lines, using SMARCA1 antibody (STJ112286) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 15s.

Anti-SMARCA1 antibody (1-200) (STJ112286)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunocytochemistry analysis of SMARCA2 (green) in 293 using SMARCA2 antibody, and DAPI (blue).

Anti-SMARCA2 antibody (62-77 aa) [R02-3E2] (STJA0012593)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-F/IHC-P/ICC/IF

Reactivity :

Human

$357.08

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Western blot analysis of extracts of various cell lines, using BRG1/SMARCA4 antibody (STJ11101695) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-SMARCA4 antibody (200-300) [S5MR] (STJ11101695)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Immunofluorescence analysis of HeLa cells using BRG1/BRG1/SMARCA4 antibody (STJ113555).

Anti-SMARCA4 antibody (30-130) (STJ113555)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

$174.83 - $867.38

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Immunoprecipitation analysis of 300 Mu g extracts of 293T cells using 3 Mu g BRG1/SMARCA4 antibody (STJ25612). Western blot was performed from the immunoprecipitate using BRG1/SMARCA4 antibody (STJ25612) at a dilution of 1:1000.

Anti-SMARCA4 antibody (680-770) (STJ25612)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Confocal imaging of C2C12 cells using SMARCA5/SNF2H Rabbit monoclonal antibody (STJ11101895, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-SMARCA5 antibody (953-1052) [S5MR] (STJ11101895)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunofluorescence analysis of A549 cells using SMARCAD1 antibody (STJ28413). Blue: DAPI for nuclear staining.

Anti-SMARCAD1 antibody (1-300) (STJ28413)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Immunoprecipitation analysis of 300 Mu g extracts of HeLa cells using 3 Mu g SMARCB1/SNF5 antibody (STJ28334). Western blot was performed from the immunoprecipitate using SMARCB1/SNF5 antibody (STJ28334) at a dilution of 1:1000.

Anti-SMARCB1 antibody (50-150) (STJ28334)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Chromatin immunoprecipitation analysis of extracts of MCF7 beta-estradiol cells, using SMARCC1/BAF155 antibody (STJ11102030) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-SMARCC1 antibody (700-800) [S0MR] (STJ11102030)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse

$197.78 - $867.38

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Immunoprecipitation analysis of 150 Mu g extracts of Jurkat cells using 3 Mu g BAF57/BAF57/SMARCE1 antibody (STJ115316). Western blot was performed from the immunoprecipitate using BAF57/BAF57/SMARCE1 antibody (STJ115316) at a dilution of 1:1000.

Anti-SMARCE1 antibody (1-411) (STJ115316)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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