• Immunofluorescence analysis of paraffin-embedded Mouse cartilage using MGP Rabbit polyclonal antibody (STJ27392) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of paraffin-embedded Rat cartilage using MGP Rabbit polyclonal antibody (STJ27392) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of MGP in paraffin-embedded rat breast using MGP Rabbit polyclonal antibody (STJ27392) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of MGP in paraffin-embedded human breast cancer using MGP Rabbit polyclonal antibody (STJ27392) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from SH-SY5Y cells, using MGP Rabbit polyclonal antibody (STJ27392) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-MGP antibody (20-103) (STJ27392)

SKU:
STJ27392

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MGP (20-103) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MGP
Gene ID: 4256
Uniprot ID: MGP_HUMAN
Immunogen Region: 20-103
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-103 of human MGP (NP_000891.2).
Immunogen Sequence: YESHESMESYELNPFINRRN ANTFISPQQRWRAKVQERIR ERSKPVHELNREACDDYRLC ERYAMVYGYNAAYNRYFRKR RGTK
Post Translational Modifications Requires vitamin K-dependent gamma-carboxylation for its function.
Function Associates with the organic matrix of bone and cartilage. Thought to act as an inhibitor of bone formation.
Protein Name Matrix Gla Protein
Mgp
Cell Growth-Inhibiting Gene 36 Protein
Cellular Localisation Secreted
Alternative Antibody Names Anti-Matrix Gla Protein antibody
Anti-Mgp antibody
Anti-Cell Growth-Inhibiting Gene 36 Protein antibody
Anti-MGP antibody
Anti-MGLAP antibody
Anti-GIG36 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance