Anti-Microtubule-associated protein 1 light chain 3 gamma antibody (N-Term) (STJA0040902)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeMixed
ImmunogenA synthetic peptide (CQEEVAGIRAKF) corresponding to the N-terminal of human MAP1LC3 C conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence deriven from MAP1LC3 C protein in Macac
General Information
| Short Description | Rabbit polyclonal anti-Microtubule-associated protein 1 light chain 3 gamma (N-Term) for use in ICC, IHC-F and WB in Human samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | ICC/IHC-F/WB |
| Host | Rabbit |
| Reactivity | Human |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | Mixed |
| Conjugation | Unconjugated |
| Purification | Whole serum |
| Dilution Range | WB 1:100-1:1000IHC 1:100-1:1000 |
| Storage Instruction | Spin vial briefly before opening. Reconstitute in 100 µL sterile-filtered, ultrapure water. Centrifuge to remove any insoluble material. After reconstitution keep aliquots at-20°C for a higher stability, and at 2-8°C with an appropriate antibacterial |
Target Information
| Gene Symbol | MAP1LC3C |
| Gene ID | 440738 |
| Uniprot ID | MLP3C_HUMAN |
| Immunogen | A synthetic peptide (CQEEVAGIRAKF) corresponding to the N-terminal of human MAP1LC3 C conjugated to Blue Carrier Protein has been used as the immunogen. The peptide is homologous with the corresponding sequence deriven from MAP1LC3 C protein in Macac |
| Immunogen Region | N-Term |
| Immunogen Sequence | Human |
| Specificity | IHC and WB confirmed the specificity for MAP1LC3 C. This antibody should recognize MAP1LC3 C only and not the other forms MAP1LC3A and MAP1LC3B. Human, other species have not yet been tested. |
Additional Info
| Tissue Specificity | Most abundant in placenta, lung and ovary. |
| Post Translational Modifications | The precursor molecule is cleaved by ATG4 (ATG4A, ATG4B, ATG4C or ATG4D) to expose the glycine at the C-terminus and form the cytosolic form, LC3-I. The processed form is then activated by APG7L/ATG7, transferred to ATG3 and conjugated to phosphatidylethanolamine (PE) phospholipid to form the membrane-bound form, LC3-II. During non-canonical autophagy, the processed form is conjugated to phosphatidylserine (PS) phospholipid. ATG4 proteins also mediate the delipidation of PE-conjugated forms. In addition, ATG4B and ATG4D mediate delipidation of ATG8 proteins conjugated to PS during non-canonical autophagy. (Microbial infection) The Legionella effector RavZ is a deconjugating enzyme that hydrolyzes the amide bond between the C-terminal glycine residue and an adjacent aromatic residue in ATG8 proteins conjugated to phosphatidylethanolamine (PE), producing an ATG8 protein that is resistant to reconjugation by the host machinery due to the cleavage of the reactive C-terminal glycine. RavZ is also able to mediate delipidation of ATG8 proteins conjugated to phosphatidylserine (PS). Phosphorylation at Ser-96 and Ser-98 by TBK1 prevents interaction with ATG4 (ATG4A, ATG4B, ATG4C or ATG4D). Phosphorylation by TBK1 on autophagosomes prevents their delipidation by ATG4 and premature removal from nascent autophagosomes. |
| Function | Ubiquitin-like modifier that plays a crucial role in antibacterial autophagy (xenophagy) through the selective binding of CALCOCO2. Recruits all ATG8 family members to infecting bacteria such as S.typhimurium. May also play a role in aggrephagy, the macroautophagic degradation of ubiquitinated and aggregated proteins. |
| Protein Name | Microtubule-Associated Protein 1 Light Chain 3 GammaAutophagy-Related Protein Lc3 CAutophagy-Related Ubiquitin-Like Modifier Lc3 CMap1 Light Chain 3-Like Protein 3Microtubule-Associated Proteins 1a/1b Light Chain 3cMap1a/Map1b Lc3 CMap1a/Map1b Light Chain 3 C |
| Database Links | Reactome: R-HSA-1632852 |
| Cellular Localisation | Cytoplasmic VesicleAutophagosome MembraneLipid-AnchorEndomembrane SystemCytoplasmCytoskeletonLc3-Ii Binds To The Autophagic Membranes |
| Alternative Antibody Names | Anti-Microtubule-Associated Protein 1 Light Chain 3 Gamma antibodyAnti-Autophagy-Related Protein Lc3 C antibodyAnti-Autophagy-Related Ubiquitin-Like Modifier Lc3 C antibodyAnti-Map1 Light Chain 3-Like Protein 3 antibodyAnti-Microtubule-Associated Proteins 1a/1b Light Chain 3c antibodyAnti-Map1a/Map1b Lc3 C antibodyAnti-Map1a/Map1b Light Chain 3 C antibodyAnti-MAP1LC3C antibody |
Information sourced from Uniprot.org