• Western blot analysis of various lysates, using Mitofusin-1/MFN1 antibody (STJ111922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Western blot analysis of Rat heart, using Mitofusin-1/MFN1 antibody (STJ111922) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunofluorescence analysis of U2OS cells using Mitofusin-1/MFN1 rabbit polyclonal antibody (STJ111922) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MFN1 antibody (622-741) (STJ111922)

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STJ111922

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MFN1 (622-741) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MFN1
Gene ID: 55669
Uniprot ID: MFN1_HUMAN
Immunogen Region: 622-741
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 622-741 of human Mitofusin-1/MFN1 (NP_284941.2).
Immunogen Sequence: YGALYLYERLSWTTHAKERA FKQQFVNYATEKLRMIVSST SANCSHQVKQQIATTFARLC QQVDITQKQLEEEIARLPKE IDQLEKIQNNSKLLRNKAVQ LENELENFTKQFLPSSNEES
Tissue Specificity Detected in kidney and heart (at protein level). Ubiquitous. Expressed at slightly higher level in kidney and heart. Isoform 2 may be overexpressed in some tumors, such as lung cancers.
Post Translational Modifications Ubiquitinated by non-degradative ubiquitin by PRKN. Deubiquitination by USP30 inhibits mitochondrial fusion. Ubiquitinated by MARCHF5. When mitochondria are depolarized and dysfunctional, it is ubiquitinated by a SCF (SKP1-CUL1-F-box protein) E3 ubiquitin-protein ligase complex that contains FBXO7 and PRKN.
Function Mitochondrial outer membrane GTPase that mediates mitochondrial clustering and fusion. Membrane clustering requires GTPase activity. It may involve a major rearrangement of the coiled coil domains. Mitochondria are highly dynamic organelles, and their morphology is determined by the equilibrium between mitochondrial fusion and fission events. Overexpression induces the formation of mitochondrial networks (in vitro). Has low GTPase activity.
Protein Name Mitofusin-1
Fzo Homolog
Transmembrane Gtpase Mfn1
Database Links Reactome: R-HSA-5205685
Reactome: R-HSA-9013419
Reactome: R-HSA-983231
Cellular Localisation Mitochondrion Outer Membrane
Multi-Pass Membrane Protein
Isoform 2: Cytoplasm
Alternative Antibody Names Anti-Mitofusin-1 antibody
Anti-Fzo Homolog antibody
Anti-Transmembrane Gtpase Mfn1 antibody
Anti-MFN1 antibody

Information sourced from Uniprot.org

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