• Immunofluorescence analysis of L929 cells using MFI2 Rabbit polyclonal antibody (STJ28736) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using MFI2 antibody (STJ28736) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-MFI2 antibody (20-200) (STJ28736)

SKU:
STJ28736

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MFI2 (20-200) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MELTF
Gene ID: 4241
Uniprot ID: TRFM_HUMAN
Immunogen Region: 20-200
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 20-200 of human MFI2 (NP_201573.1).
Immunogen Sequence: GMEVRWCATSDPEQHKCGNM SEAFREAGIQPSLLCVRGTS ADHCVQLIAAQEADAITLDG GAIYEAGKEHGLKPVVGEVY DQEVGTSYYAVAVVRRSSHV TIDTLKGVKSCHTGINRTVG WNVPVGYLVESGRLSVMGCD VLKAVSDYFGGSCVPGAGET SYSESLCRLCRGDSSGEGVC D
Tissue Specificity Found predominantly in human melanomas and in certain fetal tissues.also found in liver, epithelium, umbilical chord, placenta and sweat gland ducts.
Function Involved in iron cellular uptake. Seems to be internalized and then recycled back to the cell membrane. Binds a single atom of iron per subunit. Could also bind zinc.
Protein Name Melanotransferrin
Melanoma-Associated Antigen P97
Cd Antigen Cd228
Database Links Reactome: R-HSA-163125
Reactome: R-HSA-381426
Reactome: R-HSA-8957275
Cellular Localisation Isoform 1: Cell Membrane
Lipid-Anchor
Gpi-Anchor
Alternative Antibody Names Anti-Melanotransferrin antibody
Anti-Melanoma-Associated Antigen P97 antibody
Anti-Cd Antigen Cd228 antibody
Anti-MELTF antibody
Anti-MAP97 antibody
Anti-MFI2 antibody

Information sourced from Uniprot.org

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