| Host: |
Rabbit |
| Applications: |
WB/IHC/IF |
| Reactivity: |
Human/Mouse/Rat |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-METTL4 (1-190) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
PBS with 0.05% Proclin300, 50% Glycerol, pH7.3. |
| Purification: |
Affinity purification |
| Dilution Range: |
WB 1:1000-1:2000IHC-P 1:50-1:200IF/ICC 1:50-1:200 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
METTL4 |
| Gene ID: |
64863 |
| Uniprot ID: |
METL4_HUMAN |
| Immunogen Region: |
1-190 |
| Immunogen: |
Recombinant fusion protein containing a sequence corresponding to amino acids 1-190 of human METTL4 (NP_073751.3). |
| Immunogen Sequence: |
MSVVHQLSAGWLLDHLSFIN KINYQLHQHHEPCCRKKEFT TSVHFESLQMDSVSSSGVCA AFIASDSSTKPENDDGGNYE MFTRKFVFRPELFDVTKPYI TPAVHKECQQSNEKEDLMNG VKKEISISIIGKKRKRCVVF NQGELDAMEYHTKIRELILD GSLQLIQEGLKSGFLYPLFE KQDKGSKPIT |
| Function | N(6)-adenine-specific methyltransferase that can methylate both RNAs and DNA. Acts as a N(6)-adenine-specific RNA methyltransferase by catalyzing formation of N6,2'-O-dimethyladenosine (m6A(m)) on internal positions of U2 small nuclear RNA (snRNA): methylates the 6th position of adenine residues with a pre-deposited 2'-O-methylation. Internal m6A(m) methylation of snRNAs regulates RNA splicing. Also able to act as a N(6)-adenine-specific DNA methyltransferase by mediating methylation of DNA on the 6th position of adenine (N(6)-methyladenosine). The existence of N(6)-methyladenosine (m6A) on DNA is however unclear in mammals, and additional evidences are required to confirm the role of the N(6)-adenine-specific DNA methyltransferase activity of METTL4 in vivo. Acts as a regulator of mitochondrial transcript levels and mitochondrial DNA (mtDNA) copy number by mediating mtDNA N(6)-methylation: m6A on mtDNA reduces transcription by repressing TFAM DNA-binding and bending. N(6)-methyladenosine deposition by METTL4 regulates Polycomb silencing by triggering ubiquitination and degradation of sensor proteins ASXL1 and MPND, leading to inactivation of the PR-DUB complex and subsequent preservation of Polycomb silencing. |
| Protein Name | N(6-Adenine-Specific Methyltransferase Mettl4Methyltransferase-Like Protein 4N(6-Adenine-Specific Dna Methyltransferase Mettl4Snrna2'-O-Methyladenosine-N(6--Methyltransferase Mettl4 |
| Cellular Localisation | NucleusCytoplasmCytosolMitochondrion Matrix |
| Alternative Antibody Names | Anti-N(6-Adenine-Specific Methyltransferase Mettl4 antibodyAnti-Methyltransferase-Like Protein 4 antibodyAnti-N(6-Adenine-Specific Dna Methyltransferase Mettl4 antibodyAnti-Snrna antibodyAnti-2'-O-Methyladenosine-N(6--Methyltransferase Mettl4 antibodyAnti-METTL4 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
![Western blot analysis of lysates from wild type (WT) and ATF2 knockout (KO) 293T cells, using [KO Validated] ATF2 Rabbit polyclonal antibody (STJ22702) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/300x300/products/99177/383848/STJ22702_1__38112.1713152438.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and ATF2 knockout (KO) 293T cells, using [KO Validated] ATF2 Rabbit polyclonal antibody (STJ22702) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.")