• Western blot analysis of extracts of various cell lines, using MKL1 antibody (STJ110802) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.
  • Immunohistochemistry analysis of paraffin-embedded human liver cancer using MKL1 rabbit polyclonal antibody (STJ110802) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human colon carcinoma using MKL1 rabbit polyclonal antibody (STJ110802) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of U-2 OS cells using MKL1 Polyclonal Antibody (STJ110802) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-MKL1 antibody (1-280) (STJ110802)

SKU:
STJ110802

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Host: Rabbit
Applications: WB/IHC/IF/IP
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-MKL1 (1-280) is suitable for use in Western Blot, Immunohistochemistry, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
IP 1:20-1:50
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MRTFA
Gene ID: 57591
Uniprot ID: MRTFA_HUMAN
Immunogen Region: 1-280
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-280 of human MKL1 (NP_065882.1).
Immunogen Sequence: MPPLKSPAAFHEQRRSLERA RTEDYLKRKIRSRPERSELV RMHILEETSAEPSLQAKQLK LKRARLADDLNEKIAQRPGP MELVEKNILPVESSLKEAII VGQVNYPKVADSSSFDEDSS DALSPEQPASHESQGSVPSP LEARVSEPLLSATSASPTQV VSQLPMGRDSREMLFLAEQP PLPPPPLLPPSLTNGTTIPT AKSTPTLIKQSQPKSASEKS QRSKKAKELKPKVKKLKYH
Tissue Specificity Ubiquitously expressed, has been detected in lung, placenta, small intestine, liver, kidney, spleen, thymus, colon, muscle, heart and brain. Expressed in peripheral blood mononuclear cells (at protein level).
Post Translational Modifications Phosphorylation at Ser-6 by Erk inhibits binding of globular actin (G-actin), unmasking the nuclear localization signal (NLS) and promoting nuclear import.
Function Transcription coactivator that associates with the serum response factor (SRF) transcription factor to control expression of genes regulating the cytoskeleton during development, morphogenesis and cell migration. The SRF-MRTFA complex activity responds to Rho GTPase-induced changes in cellular globular actin (G-actin) concentration, thereby coupling cytoskeletal gene expression to cytoskeletal dynamics. MRTFA binds G-actin via its RPEL repeats, regulating activity of the MRTFA-SRF complex. Activity is also regulated by filamentous actin (F-actin) in the nucleus.
Protein Name Myocardin-Related Transcription Factor A
Mrtf-A
Mkl/Myocardin-Like Protein 1
Megakaryoblastic Leukemia 1 Protein
Megakaryocytic Acute Leukemia Protein
Database Links Reactome: R-HSA-3899300
Reactome: R-HSA-5663220
Cellular Localisation Cytoplasm
Nucleus
Subcellular Location Is Tightly Regulated By Actin Both In Cytoplasm And Nucleus: High Levels Of G-Actin In The Nucleus Observed During Serum Deprivation Lead To Low Levels Of Nuclear Mrtfa
While Reduced Levels Of Nuclear G-Actin Result In Accumulation Of Mrtfa In The Nucleus
G-Actin-Binding In The Cytoplasm Inhibits Nuclear Import By Masking The Nuclear Localization Signal (Nls)
In Contrast
Binding To Nuclear Globular Actin (G-Actin) Promotes Nuclear Export To The Cytoplasm
Nuclear Localization Is Regulated By Mical2
Which Mediates Depolymerization Of Nuclear Actin
Which Decreases Nuclear G-Actin Pool
Thereby Promoting Retention Of Mrtfa In The Nucleus And Subsequent Formation Of An Active Complex With Srf
Alternative Antibody Names Anti-Myocardin-Related Transcription Factor A antibody
Anti-Mrtf-A antibody
Anti-Mkl/Myocardin-Like Protein 1 antibody
Anti-Megakaryoblastic Leukemia 1 Protein antibody
Anti-Megakaryocytic Acute Leukemia Protein antibody
Anti-MRTFA antibody
Anti-KIAA1438 antibody
Anti-MAL antibody
Anti-MKL1 antibody

Information sourced from Uniprot.org

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