• Confocal imaging of paraffin-embedded Human liver using MGST1 Rabbit monoclonal antibody (STJ11102719, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform high pressure antigen retrieval with 0. 01 M citrate buffer (pH 6. 0) prior to IF staining.
  • Confocal imaging of NIH/3T3 cells using MGST1 Rabbit monoclonal antibody (STJ11102719, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) ( dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
  • Immunohistochemistry analysis of MGST1 in paraffin-embedded mouse kidney tissue using MGST1 Rabbit monoclonal antibody (STJ11102719) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MGST1 in paraffin-embedded mouse intestin tissue using MGST1 Rabbit monoclonal antibody (STJ11102719) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MGST1 in paraffin-embedded human thyroid cancer tissue using MGST1 Rabbit monoclonal antibody (STJ11102719) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MGST1 in paraffin-embedded human liver tissue using MGST1 Rabbit monoclonal antibody (STJ11102719) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Immunohistochemistry analysis of MGST1 in paraffin-embedded human liver cancer tissue using MGST1 Rabbit monoclonal antibody (STJ11102719) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of lysates from HepG2 cells, using MGST1 Rabbit monoclonal antibody (STJ11102719) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of various lysates using MGST1 Rabbit monoclonal antibody (STJ11102719) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-MGST1 antibody (1-100) [S9MR] (STJ11102719)

SKU:
STJ11102719

£212.50 - £501.50
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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit monoclonal antibody anti-MGST1 (1-100) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Monoclonal
Clone ID: S9MR
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 0.05% BSA, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:1000
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MGST1
Gene ID: 4257
Uniprot ID: MGST1_HUMAN
Immunogen Region: 1-100
Immunogen: A synthetic peptide corresponding to a sequence within amino acids 1-100 of human MGST1 (P10620).
Immunogen Sequence: MVDLTQVMDDEVFMAFASYA TIILSKMMLMSTATAFYRLT RKVFANPEDCVAFGKGENAK KYLRTDDRVERVRRAHLNDL ENIIPFLGIGLLYSLSGPDP
Tissue Specificity Highly expressed in liver.
Function Conjugation of reduced glutathione to a wide number of exogenous and endogenous hydrophobic electrophiles.
Protein Name Microsomal Glutathione S-Transferase 1
Microsomal Gst-1
Microsomal Gst-I
Database Links Reactome: R-HSA-156590
Reactome: R-HSA-5423646
Reactome: R-HSA-6798695
Cellular Localisation Endoplasmic Reticulum Membrane
Multi-Pass Membrane Protein
Mitochondrion Outer Membrane
Alternative Antibody Names Anti-Microsomal Glutathione S-Transferase 1 antibody
Anti-Microsomal Gst-1 antibody
Anti-Microsomal Gst-I antibody
Anti-MGST1 antibody
Anti-GST12 antibody
Anti-MGST antibody

Information sourced from Uniprot.org

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