Anti-MGRN1 antibody (1-538) (STJ11103981)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal E3 Ubiquitin-Protein Ligase Mgrn1 (1-538) antibody for use in WB, IF, ICC and ELISA in mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.05% Proclin300, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | MGRN1 |
| Gene ID | 23295 |
| Uniprot ID | MGRN1_HUMAN |
| Immunogen Region | 1-538 |
| Immunogen Sequence | MGSILSRRIAGVEDIDIQAN SAYRYPPKSGNYFASHFFMG GEKFDTPHPEGYLFGENMDL NFLGSRPVQFPYVTPAPHEP VKTLRSLVNIRKDSLRLVRY KDDADSPTEDGDKPRVLYSL EFTFDADARVAITIYCQASE EFLNGRAVYSPKSPSLQSET VHYKRGVSQQFSLPSFKIDF SEWKDDELNFDLDRGVFPVV IQAVVDEGDVVEVTGHAHVL LAAFEKHMDGSFSVKPLKQ |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-538 of human MGRN1 (O60291). |
Additional Info
| Post Translational Modifications | Autoubiquitinated in vitro. |
| Function | E3 ubiquitin-protein ligase. Mediates monoubiquitination at multiple sites of TSG101 in the presence of UBE2D1, but not of UBE2G1, nor UBE2H. Plays a role in the regulation of endosome-to-lysosome trafficking. Impairs MC1R- and MC4R-signaling by competing with GNAS-binding to MCRs and inhibiting agonist-induced cAMP production. Does not inhibit ADRB2-signaling. Does not promote MC1R ubiquitination. Acts also as a negative regulator of hedgehog signaling. |
| Protein Name | E3 Ubiquitin-Protein Ligase Mgrn1Mahogunin Ring Finger Protein 1Ring Finger Protein 156Ring-Type E3 Ubiquitin Transferase Mgrn1 |
| Database Links | Reactome: R-HSA-983168 |
| Cellular Localisation | Early EndosomeThe Endosomal Localization Is Dependent On The Interaction With Tsg101Isoform 1: CytoplasmCytosolNucleusTranslocation From The Cytosol To The Nucleus Is Seen Only In The Presence Of Mc1r And Mc4rBut Not Tbxa2rExcluded From NucleoliIsoform 2: CytoplasmIsoform 3: CytoplasmCell MembraneIsoform 4: Cytoplasm |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Mgrn1 antibodyAnti-Mahogunin Ring Finger Protein 1 antibodyAnti-Ring Finger Protein 156 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Mgrn1 antibodyAnti-MGRN1 antibodyAnti-KIAA0544 antibodyAnti-RNF156 antibody |
Information sourced from Uniprot.org