Primary Antibodies | St John's Laboratory

Immunohistochemistry analysis of paraffin-embedded Human tonsil using Phospho-Histone H3-T32 Rabbit pAb (STJ11103719) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0. 01M Tris/EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-Phospho-Histone H3-T32 antibody (STJ11103719)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat/Other

£129.50 - £642.50

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Western blot analysis of extracts of normal 293T cells and 293T transfected with GFP-tagged fusion protein, using Rabbit anti GFP-Tag pAb (STJ11103701) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-GFP-Tag antibody (1-238) (STJ11103701)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC

Reactivity :

Species independent

£135.50 - £249.50

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Western blot analysis of various lysates using PFKP Rabbit pAb (STJ11103617) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-PFKP antibody (336-540) (STJ11103617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates using IL1 Beta Rabbit pAb (STJ11103361) at 1:1000 dilution. THP-1 cells were treated by LPS (1 Mu g/ml) at 37 °C for 8 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 180s.

Anti-Il1b antibody (118-269) (STJ11103361)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of IL18 in paraffin-embedded rat spleen using IL18 Rabbit polyclonal antibody (STJ11103326) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-IL18 antibody (36-192) (STJ11103326)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U2OS cells using NDUFB8 Rabbit polyclonal antibody (STJ11103314) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-NDUFB8 antibody (29-186) (STJ11103314)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunohistochemistry analysis of paraffin-embedded rat stomach using Slc39a11 Rabbit polyclonal antibody (STJ11103273) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-Slc39a11 antibody (101-174) (STJ11103273)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using SLC5A2 rabbit polyclonal antibody (STJ11103251) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 45s.

Anti-SLC5A2 antibody (564-624) (STJ11103251)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of C6 cells using LLGL1 Rabbit polyclonal antibody (STJ11103225) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-LLGL1 antibody (1-190) (STJ11103225)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using PDE4A antibody (STJ11103193) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-PDE4A antibody (325-410) (STJ11103193)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using DNMT1 antibody (STJ11102767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-DNMT1 antibody (1-124) (STJ11102767)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of NIH/3T3 cells using TMPRSS2 Rabbit polyclonal antibody (STJ26191) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-TMPRSS2 antibody (1-100) (STJ26191)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of mouse brain cells using MAP2 Rabbit polyclonal antibody (STJ24462) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-Map2 antibody (1510-1827) (STJ24462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of various lysates, using RANKL Rabbit pAb (STJ115528) at 1:600 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-TNFSF11 antibody (78-317) (STJ115528)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Chromatin immunoprecipitation analysis of extracts of K-562 cells, using TFAM antibody (STJ115513) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-TFAM antibody (100-200) (STJ115513)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA/ChIP

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of lysates from HeLa cells using cGAS Rabbit pAb (STJ110633) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-MB21D1 antibody (350-522) (STJ110633)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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IHC on rat spinal cord using Rabbit antibody to VPS45A (STJ13100462) at a concentration of 10 ug/ml. Pre-absorption of the antibody with the immunising peptide completely abolishes the immunostaining (not shown) .

Anti-VPS45A antibody (STJ13100462)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse

£331.50

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IF (confocal) on rat brain at a concentration of 30 ug/ml using Rabbit antibody to MAP2 (STJ13100172) , DAPI counterstained.

Anti-MAP2 antibody (350-400) (STJ13100172)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse

£331.50

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Human Melanoma cell line C 32 was cultured overnight on round cover slides placed in a 24 well tissue culture plate. Culture media removed and washed twice with PBS before fixing with 2% formalin for 10 minutes. Cells were then washed three times with PBS and incubated with Tris 0. 01M containing Triton X 0. 005% for 15 minutes. Cells were washed and incubated with 100 ul of Rabbit antibody to extracellular domain of human CD36 (Fatty acid translocase) : IgG (STJ13100092) diluted 1: 100 in the blocking buffer for 30 minutes. Welles were then washed 7 times with PBS and incubated with 100 ul of anti Rb-FITC conjugate diluted 1: 100 in the blocking buffer for further 30 minutes. Cells were washed as before and nuclear counter stained with Hoechst and mounted on to slides.

Anti-CD36 antibody (ECD) (STJ13100092)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

£331.50

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STJ73128 (0. 01µg/ml) staining of Jurkat lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-AIFM1 antibody (C-Term) (STJ73128)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Mouse/Rat/Dog/Pig/Cow

£312.50

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STJ71828 (1µg/ml) staining of Mouse Kidney lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Thrombospondin antibody (N-Term) (STJ71828)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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STJ71692 (0. 5µg/ml) staining of Mouse Adipose lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-Monoglyceride Lipase antibody (Internal) (STJ71692)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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STJ70842 (0. 1µg/ml) staining of Human Liver (A) , (0. 03ug/ml) Testes (B) and Tonsil (C) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-GAPDH antibody (Internal) (STJ70842)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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STJ70667 (0. 2µg/ml) staining of HumanThymus (A) and (0. 1ug/ml) Human Spleen (B) and Mouse Thymus © lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-MYD88 antibody (Internal) (STJ70667)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF/FC

Reactivity :

Human/Mouse/Rat/Dog

£312.50

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STJ70506 (0. 03µg/ml) staining of Human (A) Mouse (B) and Rat (C) Brain lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-VPS35/MEM3 antibody (C-Term) (STJ70506)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC/IF

Reactivity :

Human/Mouse/Rat/Cow

£312.50

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STJ70381 (0. 1µg/ml) staining of HepG2 (A) and (0. 3ug/ml) MCF7 (B) nuclear cell lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-FOXA1/HNF3A antibody (C-Term) (STJ70381)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Pig/Cow

£312.50

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STJ70052 (0. 05µg/ml) staining of Human Lymph Node lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-CD20/MS4A1 antibody (C-Term) (STJ70052)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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STJ70014 (8µg/ml) staining of paraffin embedded Human Kidney. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

Anti-SOCS1 antibody (C-Term) (STJ70014)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/FC/IF/IHC

Reactivity :

Human/Mouse/Dog/Cow/Rat/Pig

£312.50

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Anti-CD5 antibody at 1:1000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-CD5 antibody (105-210aa) (STJ140164)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Anti-Rab3a antibody at 1:1000 dilution; lysates at 50 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-RAB3A antibody (181aa C-Term) (STJ140151)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Anti-ERBB1 antibody at 1:2500 dilution 50 µg of total protein per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-EGFR antibody (1162aa C-Term) (STJ140129)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£135.50 - £394.50

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Endogenous CDH1 detected with anti-CDH1 at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-FZR1 antibody (601 to 701) (STJ140099)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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Endogenous CD31 detected at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-PECAM1 antibody (690aa C-Term) (STJ140082)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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Anti-Rab27 pan antibody at 1:500 dilution 50 µg of total protein per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-RAB27A antibody (120aa C-Term) (STJ140074)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF/ELISA

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£86.50 - £534.50

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Anti-Rab5b antibody at 1:500 dilution 293 cells transfected with GFP-Rab5 lysates at 50 µg per lane rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-RAB5B antibody (115aa C-Term) (STJ140061)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Endogenous CD45 detected with at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-PTPRC antibody (1260aa C-Term) (STJ140026)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£86.50 - £534.50

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Endogenous CD19 detected with at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-CD19 antibody (510aa C-Term) (STJ140024)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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Endogenous CD4 detected at 1:500 dilution; lysate at 100 µg per lane and rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution.

Anti-CD4 antibody (50-235aa ECD) (STJ140023)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£99.50 - £373.50

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Anti-CAV2 at 1:1000 dilution; 50 µg lysate per lane of transfected HEK293 and 100 µg of HaCat cell line; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-CAV2 antibody (50aa N-Term) (STJ140020)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Western blot analysis of 293T Hela lysate, antibody was diluted at 2000. Secondary antibody was diluted at 1:20000

Anti-CDK6 antibody (280-325 aa) (STJ98721)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of 3T3 cells using Nanos Homologue 1 (NANOS1) Polyclonal Antibody diluted at 1:1500. Secondary antibody was diluted at 1:20000

Anti-NANOS1 antibody (151-200 aa) (STJ98619)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of Hela cells using ETBR Polyclonal Antibody diluted at 1:500. Secondary antibody was diluted at 1:20000

Anti-EDNRB antibody (31-80 aa) (STJ98617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of HeLa, HepG2 cells using Acetyl-NF Kappa B-p65 (K314/K315) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-RELA-Lys314/Lys315 antibody (STJ97358)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of HeLa cells using Acetyl-Ub (K27) Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-Acetyl-Ub-Lys27 antibody (STJ97356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of KB cells using CD244 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CD244 antibody (101-150 aa) (STJ97310)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of HeLa cells using EMMPRIN Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-BSG antibody (221-270 aa) (STJ96836)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of PC12 cells using FOXP3 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-FOXP3 antibody (381-430 aa) (STJ96811)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Pig

£48.00 - £252.00

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Western blot analysis of MCF7 cells using MAPK Organizer 1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-WDR83 antibody (141-190 aa) (STJ96810)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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