• Western blot analysis of lysates from NIH/3T3 cells and HepG2 cells, using Histone H3.1 (Phospho-Ser10) Antibody. The lane on the right is blocked with the phospho peptide.
  • Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using Histone H3.1 (Phospho-Ser10) Antibody. The picture on the right is blocked with the phospho peptide.
  • Western blot analysis of various cells using Phospho-Histone H3 (S10) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Western blot analysis of KB cells using Phospho-Histone H3 (S10) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).
  • Immunofluorescence analysis of Hela cell. 1, Histone H3 (phospho Ser10) Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). Beta-Tubulin monoclonal antibody (5G3) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-Phospho-Histone H3-Ser10 antibody (1-50 aa) (STJ90289)

SKU:
STJ90289

Current Stock:
Host: Rabbit
Applications: WB/IHC/IHC-F/FC/ICC/IF/ELISA
Reactivity: Human/Mouse/Rat/D.melanogaster
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-Phospho-Histone H3.1 and Histone H3.2 and Histone H3.3-Ser10 (1-50 aa) is suitable for use in Western Blot, Immunohistochemistry, Flow Cytometry, Immunocytochemistry, Immunofluorescence and ELISA research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: WB 1:500-1:2000
IHC 1:100-1:300
ICC IF 1:200-1:1000
ELISA 1:10000
IHC-fFC 1:500
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: H3C1.H3C2.H3C3.H3C4.H3C6.H3C7.H3C8.H3C10.H3C11.H3C12
8350/8351/8352/8353/8354/8355/8356/8357/8358/8968
1
Uniprot ID: H31_HUMAN
H32_HUMAN
H33_HUMAN
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Immunogen Region: 1-50 aa
Specificity: Phospho-Histone H3 (S10) Polyclonal Antibody detects endogenous levels of Histone H3 protein only when phosphorylated at S10.
Immunogen: The antiserum was produced against synthesized peptide derived from the human Histone H3.1 around the phosphorylation site of Ser10 at the amino acid range 1-50
Function Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
Protein Name Histone H3.1
Histone H3/A
Histone H3/B
Histone H3/C
Histone H3/D
Histone H3/F
Histone H3/H
Histone H3/I
Histone H3/J
Histone H3/K
Histone H3/L
Database Links Reactome: R-HSA-1266695
Reactome: R-HSA-1912408
Reactome: R-HSA-201722
Reactome: R-HSA-212300
Reactome: R-HSA-2299718
Reactome: R-HSA-2559580
Reactome: R-HSA-2559582
Reactome: R-HSA-3214815
Reactome: R-HSA-3214841
Reactome: R-HSA-3214842
Reactome: R-HSA-3214847
Reactome: R-HSA-3214858
Reactome: R-HSA-3247509
Reactome: R-HSA-427359
Reactome: R-HSA-427389
Reactome: R-HSA-427413
Reactome: R-HSA-5250924
Reactome: R-HSA-5334118
Reactome: R-HSA-5578749
Reactome: R-HSA-5617472
Reactome: R-HSA-5625886
Reactome: R-HSA-73728
Reactome: R-HSA-73772
Reactome: R-HSA-8936459
Reactome: R-HSA-8939236
Reactome: R-HSA-9018519
Reactome: R-HSA-912446
Reactome: R-HSA-9609690
Reactome: R-HSA-9610379
Reactome: R-HSA-9616222
Reactome: R-HSA-977225
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Chromosome
Alternative Antibody Names Anti-Histone H3.1 antibody
Anti-Histone H3/A antibody
Anti-Histone H3/B antibody
Anti-Histone H3/C antibody
Anti-Histone H3/D antibody
Anti-Histone H3/F antibody
Anti-Histone H3/H antibody
Anti-Histone H3/I antibody
Anti-Histone H3/J antibody
Anti-Histone H3/K antibody
Anti-Histone H3/L antibody
Anti-H3C1 antibody
Anti-H3FA antibody
Anti-HIST1H3A.H3C2 antibody
Anti-H3FL antibody
Anti-HIST1H3B.H3C3 antibody
Anti-H3FC antibody
Anti-HIST1H3C.H3C4 antibody
Anti-H3FB antibody
Anti-HIST1H3D.H3C6 antibody
Anti-H3FD antibody
Anti-HIST1H3E.H3C7 antibody
Anti-H3FI antibody
Anti-HIST1H3F.H3C8 antibody
Anti-H3FH antibody
Anti-HIST1H3G.H3C10 antibody
Anti-H3FK antibody
Anti-HIST1H3H.H3C11 antibody
Anti-H3FF antibody
Anti-HIST1H3I.H3C12 antibody
Anti-H3FJ antibody
Anti-HIST1H3J antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance