• Immunofluorescence analysis of U2OS cells using [KO Validated] macroH2A. 1 Rabbit polyclonal antibody (STJ29125). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of macroH2A. 1 in paraffin-embedded mouse lung cancer using [KO Validated] macroH2A. 1 Rabbit polyclonal antibody (STJ29125) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of macroH2A. 1 in paraffin-embedded human stomach using [KO Validated] macroH2A. 1 Rabbit polyclonal antibody (STJ29125) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of macroH2A. 1 in paraffin-embedded human kidney cancer using [KO Validated] macroH2A. 1 Rabbit polyclonal antibody (STJ29125) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of macroH2A. 1 in paraffin-embedded human kidney using [KO Validated] macroH2A. 1 Rabbit polyclonal antibody (STJ29125) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of lysates from wild type (WT) and macroH2A. 1 knockout (KO) 293T cells, using [KO Validated] macroH2A. 1 Rabbit polyclonal antibody (STJ29125) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.

Anti-H2AFY antibody (123-372) (STJ29125)

SKU:
STJ29125

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Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-macroH2A.1 (123-372) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
IF/ICC 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: MACROH2A1
Gene ID: 9555
Uniprot ID: H2AY_HUMAN
Immunogen Region: 123-372
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 123-372 of human macroH2A.1 (NP_613258.2).
Immunogen Sequence: KLEAIITPPPAKKAKSPSQK KPVSKKAGGKKGARKSKKKQ GEVSKAASADSTTEGTPADG FTVLSTKSLFLGQKLNLIHS EISNLAGFEVEAIINPTNAD IDLKDDLGNTLEKKGGKEFV EAVLELRKKNGPLEVAGAAV SAGHGLPAKFVIHCNSPVWG ADKCEELLEKTVKNCLALAD DKKLKSIAFPSIGSGRNGFP KQTAAQLILKAISSYFVSTM SSSIKTVYFVLFDSESIGI
Tissue Specificity Widely expressed.
Post Translational Modifications Monoubiquitinated at either Lys-116 or Lys-117. May also be polyubiquitinated. Ubiquitination is mediated by the CUL3/SPOP E3 complex and does not promote proteasomal degradation. Instead, it is required for enrichment in inactive X chromosome chromatin.
Function Variant histone H2A which replaces conventional H2A in a subset of nucleosomes where it represses transcription. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling. Involved in stable X chromosome inactivation. Inhibits the binding of transcription factors, including NF-kappa-B, and interferes with the activity of remodeling SWI/SNF complexes. Inhibits histone acetylation by EP300 and recruits class I HDACs, which induces a hypoacetylated state of chromatin. Isoform 1: Isoform that specifically binds poly-ADP-ribose and O-acetyl-ADP-ribose and plays a key role in NAD(+) metabolism. Able to bind to the ends of poly-ADP-ribose chains created by PARP1 and cap them. This prevents PARP1 from further addition of ADP-ribose and thus limits the consumption of nuclear NAD(+), allowing the cell to maintain proper NAD(+) levels in both the nucleus and the mitochondria to promote proper mitochondrial respiration. Increases the expression of genes involved in redox metabolism, including SOD3. Isoform 2: In contrast to isoform 1, does not bind poly-ADP-ribose. Represses SOD3 gene expression.
Protein Name Core Histone Macro-H2a.1
Histone Macroh2a1
Mh2a1
Histone H2a.y
H2a/Y
Medulloblastoma Antigen Mu-Mb-50.205
Cellular Localisation Nucleus
Chromosome
Enriched In Inactive X Chromosome Chromatin And In Senescence-Associated Heterochromatin
Recruited To Dna Damage Sites In An Aplf-Dependent Manner
Alternative Antibody Names Anti-Core Histone Macro-H2a.1 antibody
Anti-Histone Macroh2a1 antibody
Anti-Mh2a1 antibody
Anti-Histone H2a.y antibody
Anti-H2a/Y antibody
Anti-Medulloblastoma Antigen Mu-Mb-50.205 antibody
Anti-MACROH2A1 antibody
Anti-H2AFY antibody

Information sourced from Uniprot.org

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