• Western blot analysis of mouse heart, using TEAD1 antibody (STJ28851) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
  • Western blot analysis of mouse lung, using TEAD1 antibody (STJ28851) at 1:400 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunohistochemistry analysis of paraffin-embedded human liver using TEAD1 rabbit polyclonal antibody (STJ28851) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded human lung using TEAD1 rabbit polyclonal antibody (STJ28851) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.
  • Immunofluorescence analysis of MCF7 cells using TEAD1 antibody (STJ28851).
  • Chromatin immunoprecipitation analysis of extracts of HeLa cells, using TEAD1 antibody (STJ28851) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Anti-TEAD1 antibody (135-215) (STJ28851)

SKU:
STJ28851

Shipping:
Free Shipping
Current Stock:
Host: Rabbit
Applications: WB/IHC/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-TEAD1 (135-215) is suitable for use in Western Blot, Immunohistochemistry and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.05% Proclin300, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:100-1:500
IHC-P 1:50-1:200
IF/ICC 1:50-1:200
ChIP 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: TEAD1
Gene ID: 7003
Uniprot ID: TEAD1_HUMAN
Immunogen Region: 135-215
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 135-215 of human TEAD1 (NP_068780.2).
Immunogen Sequence: AIHNKLGLPGIPRPTFPGAP GFWPGMIQTGQPGSSQDVKP FVQQAYPIQPAVTAPIPGFE PASAPAPSVPAWQGRSIGTT K
Tissue Specificity Preferentially expressed in skeletal muscle. Lower levels in pancreas, placenta, and heart.
Function Transcription factor which plays a key role in the Hippo signaling pathway, a pathway involved in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein MST1/MST2, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Acts by mediating gene expression of YAP1 and WWTR1/TAZ, thereby regulating cell proliferation, migration and epithelial mesenchymal transition (EMT) induction. Binds specifically and cooperatively to the SPH and GT-IIC 'enhansons' (5'-GTGGAATGT-3') and activates transcription in vivo in a cell-specific manner. The activation function appears to be mediated by a limiting cell-specific transcriptional intermediary factor (TIF). Involved in cardiac development. Binds to the M-CAT motif.
Protein Name Transcriptional Enhancer Factor Tef-1
Ntef-1
Protein Gt-Iic
Tea Domain Family Member 1
Tead-1
Transcription Factor 13
Tcf-13
Database Links Reactome: R-HSA-2032785
Reactome: R-HSA-8951671
Reactome: R-HSA-9619665
Cellular Localisation Nucleus
Alternative Antibody Names Anti-Transcriptional Enhancer Factor Tef-1 antibody
Anti-Ntef-1 antibody
Anti-Protein Gt-Iic antibody
Anti-Tea Domain Family Member 1 antibody
Anti-Tead-1 antibody
Anti-Transcription Factor 13 antibody
Anti-Tcf-13 antibody
Anti-TEAD1 antibody
Anti-TCF13 antibody
Anti-TEF1 antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance