![Immunofluorescence analysis of U2OS cells using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102871/392040/STJ29766_1__51820.1713162400.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
![Immunohistochemistry analysis of Integrin alpha 2 (ITGA2/CD49b) in paraffin-embedded human tonsil using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102871/392041/STJ29766_2__46810.1713162400.jpg?c=1 "Immunohistochemistry analysis of Integrin alpha 2 (ITGA2/CD49b) in paraffin-embedded human tonsil using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of Integrin alpha 2 (ITGA2/CD49b) in paraffin-embedded human placenta using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102871/392042/STJ29766_3__28099.1713162401.jpg?c=1 "Immunohistochemistry analysis of Integrin alpha 2 (ITGA2/CD49b) in paraffin-embedded human placenta using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Immunohistochemistry analysis of Integrin alpha 2 (ITGA2/CD49b) in paraffin-embedded human lung cancer using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102871/392043/STJ29766_4__56689.1713162402.jpg?c=1 "Immunohistochemistry analysis of Integrin alpha 2 (ITGA2/CD49b) in paraffin-embedded human lung cancer using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at dilution of 1:50 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.")
![Western blot analysis of lysates from wild type (WT) and Integrin alpha 2 (ITGA2/CD49b) knockout (KO) 293T cells, using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102871/392044/STJ29766_5__90276.1713162403.jpg?c=1 "Western blot analysis of lysates from wild type (WT) and Integrin alpha 2 (ITGA2/CD49b) knockout (KO) 293T cells, using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Western blot analysis of various lysates using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/124x110/products/102871/392045/STJ29766_6__30911.1713162405.jpg?c=1 "Western blot analysis of various lysates using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.")
![Immunofluorescence analysis of U2OS cells using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.](https://cdn11.bigcommerce.com/s-zso2xnchw9/images/stencil/1280x1280/products/102871/392040/STJ29766_1__51820.1713162400.jpg?c=1 "Immunofluorescence analysis of U2OS cells using [KO Validated] Integrin alpha 2 (ITGA2/CD49b) Rabbit polyclonal antibody (STJ29766) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.")
Anti-ITGA2 antibody (30-162) (STJ29766)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal ITGA2 (30-162) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:1000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ITGA2 |
| Gene ID | 3673 |
| Uniprot ID | ITA2_HUMAN |
| Immunogen Region | 30-162 |
| Immunogen Sequence | YNVGLPEAKIFSGPSSEQFG YAVQQFINPKGNWLLVGSPW SGFPENRMGDVYKCPVDLST ATCEKLNLQTSTSIPNVTEM KTNMSLGLILTRNMGTGGFL TCGPLWAQQCGNQYYTTGVC SDISPDFQLSASF |
| Specificity | A synthetic peptide corresponding to a sequence within amino acids 30-162 of human Integrin alpha 2 (ITGA2/CD49b) (NP_002194.2). |
Additional Info
| Function | Integrin alpha-2/beta-1 is a receptor for laminin, collagen, collagen C-propeptides, fibronectin and E-cadherin. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. It is responsible for adhesion of platelets and other cells to collagens, modulation of collagen and collagenase gene expression, force generation and organization of newly synthesized extracellular matrix. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human rotavirus A. (Microbial infection) Integrin ITGA2:ITGB1 acts as a receptor for Human echoviruses 1 and 8. |
| Protein Name | Integrin Alpha-2Cd49 Antigen-Like Family Member BCollagen ReceptorPlatelet Membrane Glycoprotein IaGpiaVla-2 Subunit AlphaCd Antigen Cd49b |
| Database Links | Reactome: R-HSA-216083Reactome: R-HSA-3000157Reactome: R-HSA-3000170Reactome: R-HSA-3000178Reactome: R-HSA-447041Reactome: R-HSA-75892Reactome: R-HSA-8874081Reactome: R-HSA-9926550 |
| Cellular Localisation | MembraneSingle-Pass Type I Membrane Protein |
| Alternative Antibody Names | Anti-Integrin Alpha-2 antibodyAnti-Cd49 Antigen-Like Family Member B antibodyAnti-Collagen Receptor antibodyAnti-Platelet Membrane Glycoprotein Ia antibodyAnti-Gpia antibodyAnti-Vla-2 Subunit Alpha antibodyAnti-Cd Antigen Cd49b antibodyAnti-ITGA2 antibodyAnti-CD49B antibody |
Information sourced from Uniprot.org