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Anti-Phospho-PKC-Thr497 antibody (623-672 aa) (STJ90387)

Western blot analysis of lysates from NIH/3T3 cells treated with PMA 250ng/ml 15', using PKC-pan (Phospho-Thr497) Antibody. The lane on the right is blocked with the phospho peptide.
Immunohistochemistry analysis of paraffin-embedded human breast carcinoma, using PKC-pan (Phospho-Thr497) Antibody. The picture on the right is blocked with the phospho peptide.
Western blot analysis of 293 cells using Phospho-PKC (T497) Polyclonal Antibody diluted at 1：500
Immunofluorescence analysis of HepG2 cells, using PKC-pan (Phospho-Thr497) Antibody. The picture on the right is blocked with the phospho peptide.
Immunofluorescence analysis of Hela cell. 1, PKC (phospho Thr497) Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-Phospho-PKC-Thr497 antibody (623-672 aa) (STJ90387)

SKU:

STJ90387

£46.00 - £226.00

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General Information
Product Properties
Target Information
Additional Info
Warranty Information

Host:	Rabbit
Applications:	WB/IHC/IF/ELISA
Reactivity:	Human/Mouse/Rat
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Rabbit polyclonal antibody anti-Phospho-Protein kinase C alpha type and Protein kinase C beta type and Protein kinase C delta type and Protein kinase C epsilon type and Protein kinase C gamma type and Protein kinase C eta type and Protein kinase C th

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification:	The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration:	1 mg/mL
Dilution Range:	WB 1:500-1:2000 IHC 1:100-1:300 IF 1:200-1:1000 ELISA 1:40000
Storage Instruction:	Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.

Gene Symbol:	PRKCQ PRKCH 5588 5583 5582 KPCT_HUMAN KPCL_HUMAN KPCG_HUMAN
Immunogen Region:	623-672 aa
Specificity:	Phospho-PKC (T497) Polyclonal Antibody detects endogenous levels of PKC protein only when phosphorylated at T497.
Immunogen:	The antiserum was produced against synthesized peptide derived from the human PKC-pan around the phosphorylation site of Thr497 at the amino acid range 623-672

Protein Name	Protein Kinase C Alpha Type Pkc-A Pkc-Alpha
Database Links	Reactome: R-HSA-111933 Reactome: R-HSA-114516 Reactome: R-HSA-1250196 Reactome: R-HSA-1433557 Reactome: R-HSA-1433559 Reactome: R-HSA-2179392 Reactome: R-HSA-2514859 Reactome: R-HSA-3000170 Reactome: R-HSA-399997 Reactome: R-HSA-4086398 Reactome: R-HSA-416993 Reactome: R-HSA-418597 Reactome: R-HSA-4419969 Reactome: R-HSA-450520 Reactome: R-HSA-5099900 Reactome: R-HSA-5218921 Reactome: R-HSA-5668599 Reactome: R-HSA-76005 Reactome: R-HSA-8853659 Reactome: R-HSA-9010642
Cellular Localisation	Cytoplasm Cell Membrane Peripheral Membrane Protein Mitochondrion Membrane Nucleus
Alternative Antibody Names	Anti-Protein Kinase C Alpha Type antibody Anti-Pkc-A antibody Anti-Pkc-Alpha antibody Anti-PRKCA antibody Anti-PKCA antibody Anti-PRKACA antibody

Information sourced from Uniprot.org

12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance

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