St John's Laboratory Ltd

Anti-BIRC6 antibody (STJA0004819)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£139.50 - £403.50

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g UBA6 antibody (STJ29647). Western blot was performed from the immunoprecipitate using UBA6 antibody (STJ29647) at a dilution of 1:1000.

Anti-UBA6 antibody (1-260) (STJ29647)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/IP/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunofluorescence analysis of PC-12 cells using USP9X/USP9Y Rabbit polyclonal antibody (STJ11103439) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-USP9Y antibody (2456-2555) (STJ11103439)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ70293 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).

Anti-E2-EPF/UBE2S antibody (N-Term) (STJ70293)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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Western blot analysis of lysates from wild type (WT) and UBE2S knockout (KO) 293T cells, using [KO Validated] UBE2S Rabbit polyclonal antibody (STJ11100037) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 5s.

Anti-UBE2S antibody (122-221) (STJ11100037)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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Anti-Recombinant-CUL3 antibody [RM2E80] (STJA0025450)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

FC/IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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STJ70374 staining (1µg/ml) of Jurkat lysate (RIPA buffer, 30µg total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.

Anti-USP11/UHX1 antibody (C-Term) (STJ70374)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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SDS-PAGE gel of recombinant human UCHL3 protein under reducing conditions stained with Coomassie blue

Human UCHL3 protein (Recombinant) (His-Tag) (STJP017010)

Host :

E.coli

Applications :

SDS-PAGE/Enzyme Activity

£134.50 - £738.50

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Anti-BIRC6/Apollon antibody (4724-4735) (STJ72374)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA

Reactivity :

Human/Mouse/Rat/Cow

£193.50

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Anti-BIRC6 antibody (STJA0004818)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/ELISA

Reactivity :

Human/Mouse

£139.50 - £403.50

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Western blot analysis of extracts of various cell lines, using USP11 Rabbit monoclonal antibody (STJ11103039) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-USP11 antibody (800-900) [S9MR] (STJ11103039)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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UCHL3 antibody western blot 293T cells STJA0035845

Anti-UCHL3 antibody [R07-1K4] (STJA0035845)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Immunohistochemistry analysis of USP47 in paraffin-embedded Human colon carcinoma using USP47 Rabbit polyclonal antibody (STJ117656) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-USP47 antibody (1128-1287) (STJ117656)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of various lysates using UCHL3 Rabbit pAb (STJ110455) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 90s.

Anti-UCHL3 antibody (1-220) (STJ110455)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of U2OS cells using USP11 Rabbit polyclonal antibody (STJ11103210) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-USP11 antibody (70-190) (STJ11103210)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using Cullin 3 Rabbit polyclonal antibody (STJ118883) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CUL3 antibody (569-768) (STJ118883)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of HeLa cells using Cullin 3 antibody (STJ23291). Blue: DAPI for nuclear staining.

Anti-CUL3 antibody (468-768) (STJ23291)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of extracts of various cell lines, using RNF168 antibody (STJ25369) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-RNF168 antibody (300-571) (STJ25369)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded mouse spleen using UBE2D1 antibody (STJ26014) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.

Anti-UBE2D1 antibody (1-147) (STJ26014)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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STJ70235 (0. 5µg/ml) staining of JURKAT lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-RNF31/ZIBRA antibody (C-Term) (STJ70235)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human/Dog/Pig

£312.50

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Immunofluorescence of HeLa cells with UBE2S antibody green and phalloidin-TRITC actin staining red

Anti-E2-EPF/UBE2S antibody (1-222aa) [AT2C12] (STJA0041579)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB/ICC/IF/FACS

Reactivity :

Human

£219.50 - £332.50

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Anti-ITCH antibody (250-300) {FITC} (STJ501508)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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Anti-ITCH antibody (250-300) {Biotin} (STJ501507)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£497.50

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ITCH/AIP4 Positive Control peptide (STJ504326)

Applications :

WB

£308.50

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SDS-PAGE gel showing recombinant human E2-EPF UBE2S protein 3 μg under reducing conditions with Coomassie blue staining

Human E2-EPF/UBE2S protein (Recombinant) (His-Tag) (STJP016514)

Host :

E.coli

Applications :

SDS-PAGE

£110.50 - £535.50

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Immunofluorescence analysis of NIH/3T3 cells using RNF149 Rabbit polyclonal antibody (STJ118075) at dilution of 1:50 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-RNF149 antibody (221-400) (STJ118075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various cells using CUL-3 Polyclonal Antibody diluted at 1：1000

Anti-CUL3 antibody (1-50 aa) (STJ92524)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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Anti-Recombinant-CUL3 antibody [RM2E81] (STJA0025451)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IP/WB

Reactivity :

Human/Mouse/Rat

£300.50 - £457.50

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Human CUL3 protein (Recombinant) (N-His) (STJP008408)

Host :

E.coli

Applications :

ELISA/Immunogen/SDS-PAGE/WB

£208.50 - £1,163.50

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Cullin 3 antibody western blot PC-12 rat spleen STJA0034209

Anti-Cullin 3 antibody [R09-4D1] (STJA0034209)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Western blot analysis of various lysates using ITCH Rabbit mAb (STJ11102343) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-ITCH antibody (1-100) [S3MR] (STJ11102343)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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ITCH/AIP4 antibody western blot A549 HL-60 STJA0035242

Anti-ITCH antibody [R03-4I6] (STJA0035242)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human

£133.50 - £281.50

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Western blot analysis of various lysates using UBE2S Rabbit pAb (STJ26022) at 1:1000 dilution incubated overnight at 4 °C. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-UBE2S antibody (1-222) (STJ26022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Immunohistochemistry analysis of paraffin-embedded human colon using UCHL3 Rabbit monoclonal antibody (STJ11101430) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM Tris/EDTA buffer pH 9. 0 before commencing with immunohistochemistry staining protocol.

Anti-UCHL3 antibody (1-100) [S0MR] (STJ11101430)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£118.50 - £521.50

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USP11 antibody western blot Jurkat rat brain HeLa STJA0033889

Anti-USP11 antibody [R08-2D7] (STJA0033889)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Rat

£133.50 - £281.50

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Human OTUB1 protein (Recombinant) (N-GST) (STJP014683)

Host :

E.coli

Applications :

ELISA/WB

£319.50

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Western blot with Anti-ITCH Antibody (STJ501506) of THP cell at 1:500 dilution in buffer.

Anti-ITCH antibody (250-300) (STJ501506)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Mouse/Rat

£400.50

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Human Atrophin-1 interacting protein 4/ITCH protein (Recombinant) (NO-Tag) (STJP020387)

Host :

E.coli

£427.50

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Western blot analysis of various lysates using RMND5A Rabbit polyclonal antibody (STJ117123) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-RMND5A antibody (200-270) (STJ117123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-TRIM32 antibody (160-210 aa) (STJ13102505)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Mouse/Rat

£322.50

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SFT antibody western blot Raw2647 STJA0012589

Anti-UBE2D1 antibody (119-132 aa) [R07-4E8] (STJA0012589)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP

Reactivity :

Human/Mouse

£133.50 - £281.50

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SDS-PAGE gel of recombinant human UbcH6 UBE2E1 protein under reducing conditions

Human UbcH6/UBE2E1 protein (Recombinant) (His-Tag) (STJP017799)

Host :

E.coli

Applications :

SDS-PAGE

£134.50 - £738.50

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Western blot analysis of lysates from wild type (WT) and ITCH knockout (KO) 293T cells, using [KO Validated] ITCH Rabbit polyclonal antibody (STJ118061) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-ITCH antibody (1-155) (STJ118061)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£126.50 - £618.50

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STJ71108 (0. 1µg/ml) staining of Human Brain (Frontal Cortex) lysate (35µg protein in RIPA buffer). Detected by chemiluminescence.

Anti-ITCH/AIF4 antibody (Internal) (STJ71108)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IF/FC

Reactivity :

Human/Mouse/Rat/Dog/Cow

£312.50

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ITCH/AIP4 Blocking Peptide peptide (STJ504270)

Applications :

Immunodepletion/Immunocompetition

£260.50

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ITCH antibody western blot K562 cells STJA0037252

Anti-ITCH antibody [R05-6P2] (STJA0037252)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P

Reactivity :

Human/Mouse/Rat

£133.50 - £281.50

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Anti-Recombinant-CUL4A antibody [RM2E83] (STJA0025453)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

IHC/IP/WB

Reactivity :

Human

£300.50 - £457.50

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Anti-CUL4A antibody (590-670 aa) (STJ191690)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£49.00 - £261.00

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