• Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g UBA6 antibody (STJ29647). Western blot was performed from the immunoprecipitate using UBA6 antibody (STJ29647) at a dilution of 1:1000.
  • Immunofluorescence analysis of A549 cells using UBA6 Rabbit polyclonal antibody (STJ29647). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Western blot analysis of various lysates using UBA6 Rabbit polyclonal antibody (STJ29647) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-UBA6 antibody (1-260) (STJ29647)

SKU:
STJ29647

€140.63 - €653.13
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Host: Rabbit
Applications: WB/IF/IP
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-UBA6 (1-260) is suitable for use in Western Blot, Immunofluorescence and Immunoprecipitation research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
IP 1:50-1:100
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: UBA6
Gene ID: 55236
Uniprot ID: UBA6_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human UBA6 (NP_060697.4).
Immunogen Sequence: MEGSEPVAAHQGEEASCSSW GTGSTNKNLPIMSTASVEID DALYSRQRYVLGDTAMQKMA KSHVFLSGMGGLGLEIAKNL VLAGIKAVTIHDTEKCQAWD LGTNFFLSEDDVVNKRNRAE AVLKHIAELNPYVHVTSSSV PFNETTDLSFLDKYQCVVLT EMKLPLQKKINDFCRSQCPP IKFISADVHGIWSRLFCDFG DEFEVLDTTGEEPKEIFISN ITQANPGIVTCLENHPHKL
Tissue Specificity Widely expressed. Isoform 2 is predominantly expressed in testis with higher expression in adult testis than in fetal testis.
Function Activates ubiquitin by first adenylating its C-terminal glycine residue with ATP, and thereafter linking this residue to the side chain of a cysteine residue in E1, yielding a ubiquitin-E1 thioester and free AMP. Specific for ubiquitin, does not activate ubiquitin-like peptides. Differs from UBE1 in its specificity for substrate E2 charging. Does not charge cell cycle E2s, such as CDC34. Essential for embryonic development. Required for UBD/FAT10 conjugation. Isoform 2 may play a key role in ubiquitin system and may influence spermatogenesis and male fertility.
Protein Name Ubiquitin-Like Modifier-Activating Enzyme 6
Ubiquitin-Activating Enzyme 6
Monocyte Protein 4
Mop-4
Ubiquitin-Activating Enzyme E1-Like Protein 2
E1-L2
Database Links Reactome: R-HSA-8866652
Reactome: R-HSA-983168
Alternative Antibody Names Anti-Ubiquitin-Like Modifier-Activating Enzyme 6 antibody
Anti-Ubiquitin-Activating Enzyme 6 antibody
Anti-Monocyte Protein 4 antibody
Anti-Mop-4 antibody
Anti-Ubiquitin-Activating Enzyme E1-Like Protein 2 antibody
Anti-E1-L2 antibody
Anti-UBA6 antibody
Anti-MOP4 antibody
Anti-UBE1L2 antibody

Information sourced from Uniprot.org

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