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Anti-E2-EPF/UBE2S antibody (N-Term) (STJ70293)

STJ70293 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
STJ70293 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0. 15% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml) , showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10ug/ml) followed by Alexa Fluor 488 secondary antibody (2ug/ml).
STJ70293 Flow cytometric analysis of paraformaldehyde fixed HeLa cells (blue line) , permeabilized with 0. 5% Triton. Primary incubation 1hr (10ug/ml) followed by Alexa Fluor 488 secondary antibody (1ug/ml). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

Anti-E2-EPF/UBE2S antibody (N-Term) (STJ70293)

SKU:

STJ70293-100

£303.50

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General Information
Product Properties
Target Information
Additional Info
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Host:	Goat
Applications:	Pep-ELISA/IF/FC
Reactivity:	Human/Mouse/Rat/Dog/Cow
Note:	STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description:	Goat polyclonal antibody anti-E2-EPF/UBE2S (N-Term) is suitable for use in ELISA, Immunofluorescence and Flow Cytometry research applications.

Clonality:	Polyclonal
Conjugation:	Unconjugated
Isotype:	IgG
Formulation:	0.5 mg/ml in Tris saline, 0.02% sodium azide, pH7.3 with 0.5% bovine serum albumin. NA
Purification:	Purified from goat serum by ammonium sulphate precipitation followed by antigen affinity chromatography using the immunizing peptide.
Concentration:	0.5 mg/mL
Dilution Range:	IF-Strong expression of the protein seen in the cytoplasm and nuclei of A431 and HeLa cells. 10µg/ml ELISA-antibody detection limit dilution 1:32000.
Storage Instruction:	Store at-20°C on receipt and minimise freeze-thaw cycles.

Gene Symbol:	UBE2S
Gene ID:	27338
Uniprot ID:	UBE2S_HUMAN
Immunogen Region:	N-Term
Accession Number:	NP_055316.2
Immunogen Sequence:	NSNVENLPPHIIR

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes 'Lys-11'-linked polyubiquitination. Acts as an essential factor of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated ubiquitin ligase that controls progression through mitosis. Acts by specifically elongating 'Lys-11'-linked polyubiquitin chains initiated by the E2 enzyme UBE2C/UBCH10 on APC/C substrates, enhancing the degradation of APC/C substrates by the proteasome and promoting mitotic exit. Also acts by elongating ubiquitin chains initiated by the E2 enzyme UBE2D1/UBCH5 in vitro.it is however unclear whether UBE2D1/UBCH5 acts as an E2 enzyme for the APC/C in vivo. Also involved in ubiquitination and subsequent degradation of VHL, resulting in an accumulation of HIF1A. In vitro able to promote polyubiquitination using all 7 ubiquitin Lys residues, except 'Lys-48'-linked polyubiquitination.
Protein Name	Ubiquitin-Conjugating Enzyme E2 S E2 Ubiquitin-Conjugating Enzyme S E2-Epf Ubiquitin Carrier Protein S Ubiquitin-Conjugating Enzyme E2-24 Kda Ubiquitin-Conjugating Enzyme E2-Epf5 Ubiquitin-Protein Ligase S
Database Links	Reactome: R-HSA-141430 Reactome: R-HSA-174048 Reactome: R-HSA-174084 Reactome: R-HSA-174154 Reactome: R-HSA-174178 Reactome: R-HSA-174184 Reactome: R-HSA-176407 Reactome: R-HSA-176408 Reactome: R-HSA-176409 Reactome: R-HSA-176412 Reactome: R-HSA-179409 Reactome: R-HSA-2467813 Reactome: R-HSA-2559582 Reactome: R-HSA-68867 Reactome: R-HSA-69017 Reactome: R-HSA-8853884 Reactome: R-HSA-8866652 Reactome: R-HSA-9687136 Reactome: R-HSA-983168
Alternative Antibody Names	Anti-Ubiquitin-Conjugating Enzyme E2 S antibody Anti-E2 Ubiquitin-Conjugating Enzyme S antibody Anti-E2-Epf antibody Anti-Ubiquitin Carrier Protein S antibody Anti-Ubiquitin-Conjugating Enzyme E2-24 Kda antibody Anti-Ubiquitin-Conjugating Enzyme E2-Epf5 antibody Anti-Ubiquitin-Protein Ligase S antibody Anti-UBE2S antibody Anti-E2EPF antibody Anti-OK antibody Anti-SW-cl.73 antibody

Information sourced from Uniprot.org

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