Anti-RNF149 antibody (221-400) (STJ118075)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal RNF149 (221-400) antibody for use in WB, IHC-P, IF, ICC and ELISA in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/ELISA |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200ELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specific assay requirements. |
| Formulation | PBS with 0.09% Sodium Azide, 50% Glycerol, pH7.3. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | RNF149 |
| Gene ID | 284996 |
| Uniprot ID | RN149_HUMAN |
| Immunogen Region | 221-400 |
| Immunogen Sequence | FYYIQRFLYTGSQIGSQSHR KETKKVIGQLLLHTVKHGEK GIDVDAENCAVCIENFKVKD IIRILPCKHIFHRICIDPWL LDHRTCPMCKLDVIKALGYW GEPGDVQEMPAPESPPGRDP AANLSLALPDDDGSDDSSPP SASPAESEPQCDPSFKGDAG ENTALLEAGRSDSRHGGPIS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 221-400 of human RNF149 (NP_775918.2). |
Additional Info
| Function | E3 ubiquitin-protein ligase. Ubiquitinates BRAF, inducing its proteasomal degradation. |
| Protein Name | E3 Ubiquitin-Protein Ligase Rnf149Dna Polymerase-Transactivated Protein 2Ring Finger Protein 149Ring-Type E3 Ubiquitin Transferase Rnf149 |
| Database Links | |
| Cellular Localisation | MembraneSingle-Pass Membrane Protein |
| Alternative Antibody Names | Anti-E3 Ubiquitin-Protein Ligase Rnf149 antibodyAnti-Dna Polymerase-Transactivated Protein 2 antibodyAnti-Ring Finger Protein 149 antibodyAnti-Ring-Type E3 Ubiquitin Transferase Rnf149 antibodyAnti-RNF149 antibodyAnti-DNAPTP2 antibody |
Information sourced from Uniprot.org