Organelle Biogenesis And Maintenance | Research Area

Western blot analysis of lysates from PC-12 cells, using Phospho-p38 MAPK-T180 Rabbit pAb (STJ11107612) at 1:2000 dilution. PC-12 cells were treated by UV for 15-30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10S.

Anti-Phospho-p38 MAPK-T180 antibody (STJ11107612)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat

$174.83 - $867.38

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Western blot analysis of lysates from HeLa cells, using Phospho-MAPK14-T180/Y182 pAb (STJ11107626) at 1:2000 dilution or MAPK14 antibody (STJ228561). HeLa cells were treated by TNF-Alpha (20 ng/mL) at 37 °C for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-Phospho-p38 MAPK-T180/Y182 antibody (STJ11107626)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Western blot analysis of lysates from 293 and NIH/3T3 cells, using Phospho-p38 MAPK-Y322 Rabbit pAb (STJ11107611) at 1:1000 dilution. 293 cells were treated by UV for 15-30 minutes. NIH/3T3 cells were treated by Anisomycin (25 Mu g/mL) for 30 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 15s.

Anti-Phospho-p38 MAPK-Y322 antibody (STJ11107611)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

$174.83 - $867.38

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Immunohistochemical analysis of paraffin-embedded human Colon cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PKD2-Ser812 antibody (778-827 aa) (STJ91356)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of PLK1 (Phospho-Ser137) Antibody. The lane on the right is blocked with the PLK1 (Phospho-Ser137) peptide.

Anti-Phospho-PLK1-Ser137 antibody (103-152 aa) (STJ90594)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA/IHC

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-PLK1-T210 antibody (STJ113485)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of lysates from HT-29 cells, using Phospho-PLK1-T210 (STJ11102606) at 1:1000 dilution. HT-29 cells were treated by nocodazole (100 ng/mL) at 37 °C for 16 hours. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 3min.

Anti-Phospho-PLK1-T210 antibody [S6MR] (STJ11102606)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$197.78 - $867.38

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Western blot analysis of 293 using p-Plk (T210) antibody.

Anti-Phospho-PLK1-Thr210 antibody (176-225 aa) (STJ91124)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-PLK4 antibody (300-350) (STJ506388)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$713.48

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Anti-Phospho-PLK4 antibody (300-350) {Biotin} (STJ506389)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$789.08

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Anti-Phospho-PLK4 antibody (300-350) {FITC} (STJ506390)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$789.08

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Western blot of rat synaptic membrane showing specific immunolabeling of the ~66 kDa PLK protein phosphorylated at Thr210 in the first lane (-). Phosphospecificity is shown in the second lane (+) where the immunolabeling is completely eliminated by blot treatment with lambda phosphatase ( Lambda-Ptase, 1200 units for 30 minutes).

Anti-Phospho-Polo-Like Kinase-Thr210 antibody (STJA0003749)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Rat/Bovine/Canine/Non-Human Primates/Mouse/Xenopus/Zebrafish

$613.58

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Immunohistochemical analysis of paraffin-embedded human liver cancer. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 45min).

Anti-Phospho-PPARA-Ser21 antibody (STJA0009830)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC

Reactivity :

Human/Rat/Mouse

$64.80 - $340.20

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Anti-Phospho-PPARA-Ser21 antibody (STJA0009917)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from 293 cells, using Phospho-AMPK Beta 1-S108 Rabbit pAb (STJ29363) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PRKAB1-S108 antibody (STJ29363)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of various cells using Phospho-AMPK Beta 1 (S182) Polyclonal Antibody cells nucleus extracted by Minute TM Cytoplasmic and Nuclear Fractionation kit (SC-003, Inventbiotech, MN, USA).

Anti-Phospho-PRKAB1-Ser182 antibody (147-196 aa) (STJ90171)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from SH-SY5Y cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-Phospho-PRKAB1-Ser182 antibody (147-196 aa) (STJ90874)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Western blot analysis of lysates from HeLa and 293 cells, using Phospho-PKA C-alpha (PRKACA) -S339 Rabbit pAb (STJ113496) at 1:1000 dilution. HeLa cells were treated by EGF (100ng/mL) for 30 minutes after serum-starvation overnight. 293T cells were treated by Insulin (100nM) for 10 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PRKACA-S339 antibody (STJ113496)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

$174.83 - $867.38

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Immunohistochemical analysis of paraffin-embedded human tonsil. 1, Antibody was diluted at 1:200 (4°C overnight). 2, Tris-EDTA, pH9.0 was used for antigen retrieval. 3, Secondary antibody was diluted at 1:200 (room temperature, 30min).

Anti-Phospho-PRKACA-Ser338 antibody (STJ91123)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of lysates from 293T cells, using Phospho-PKA C-alpha (PRKACA) -T197 Rabbit pAb (STJ113495) at 1:1000 dilution. 293T cells were treated by Insulin (100nM) for 10 minutes or treated by PMA/TPA (200nM) for 30 minutes after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA.

Anti-Phospho-PRKACA-T197 antibody (STJ113495)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $867.38

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Immunohistochemical analysis of paraffin-embedded Human colon cancer. Antibody was diluted at 1:100 (4°C overnight). High-pressure and temperature Tris-EDTA, pH8.0 was used for antigen retrieval. Negetive contrl (right) obtaned from antibody was pre-absorbed by immunogen peptide.

Anti-Phospho-PRKAR2B-Ser113 antibody (79-128 aa) (STJ90520)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$64.80 - $340.20

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Immunohistochemistry analysis of paraffin-embedded human brain, using Rhodopsin (Phospho-Ser334) Antibody. The picture on the right is blocked with the phospho peptide.

Anti-Phospho-RHO-Ser334 antibody (299-348 aa) (STJ91126)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-Phospho-SMO-S611 antibody (STJ11107677)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of lysates from Jurkat cells, using Phospho-SMO-S611/615 Rabbit pAb (STJ11101073) at 1:1000 dilution. Jurkat cells were treated by Calyculin A (100 nM) at 37 °C for 30 minutes. Jurkat cells were treated by Anisomycin (25 Mu g/mL) at 37 °C for 20 minutes. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Basic Kit <br/>Exposure time: 60s.

Anti-Phospho-SMO-S611/615 antibody (STJ11101073)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Western blot analysis of lysates from 293T cells, using Phospho-SMO-S611/615/616 Rabbit pAb (STJ11101074) at 1:1000 dilution. 293T-SMO cells were treated by SHH (0. 4 Mu g/mL) at 37 °C after serum-starvation overnight. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% BSA. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 1min.

Anti-Phospho-SMO-S611/615/616 antibody (STJ11101074)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Anti-Phospho-SMO-S615 antibody (STJ11101071)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Anti-Phospho-SMO-S615/616 antibody (STJ11107678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Dot-blot analysis of all sorts of peptides using Phospho-SMO-S616 antibody (STJ11101072) at 1:1000 dilution. <br/>Exposure time: 1s.

Anti-Phospho-SMO-S616 antibody (STJ11101072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/DB/ELISA

Reactivity :

Human

$174.83 - $867.38

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Anti-Phospho-TORC2 antibody (STJ502714)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$713.48

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Anti-Phospho-TORC2 antibody {Biotin} (STJ502715)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$789.08

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Anti-Phospho-TORC2 antibody {FITC} (STJ502716)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human/Mouse/Rat

$789.08

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Anti-Phospho-TUBA1C-Ser-165 antibody (STJA0005627)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Chicken/Human/Mouse/Rat/Xenopus

$496.13

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Anti-Phospho-TUBB3-Ser-172 antibody (STJA0005631)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ICC

Reactivity :

Chicken/Human/Mouse/Rat

$496.13

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Western blot analysis of various lysates, using PKA C-alpha (PRKACA) antibody (STJ11102940) at 1:10000 dilution. Secondary antibody: HRP Goat Anti-mouse IgG (H+L) (STJS000854) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-PKA C-alpha antibody [S0MR] (STJ11102940)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

$197.78 - $867.38

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Anti-PKD1 antibody (3700-3860 aa) (STJ11106119)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

$151.88 - $708.08

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Anti-PKD1 antibody (STJ13101565)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

$447.53

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Anti-PKD1 antibody (STJ13101566)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

$447.53

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Anti-PKD1 antibody (STJA0008526)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using PKD2 antibody (STJ25010) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-PKD2 antibody (879-968) (STJ25010)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$151.88 - $708.08

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Anti-PKD2 antibody (STJ13101578)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Human

$447.53

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Immunofluorescence analysis of A549 cells, using PLK1 Antibody. The picture on the right is blocked with the synthesized peptide.

Anti-PLK1 antibody (176-225 aa) (STJ95162)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Western blot analysis of extracts of various cell lines, using PLK1 antibody (STJ11104170) at1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60s.

Anti-PLK1 antibody (272-495) [S4170RM] (STJ11104170)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat/Monkey

$174.83 - $775.58

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Western blot analysis of lysates from Rat testis using PLK1 Rabbit monoclonal antibody (STJ11104884) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 120s.

Anti-PLK1 antibody (272-495) [S4884RM] (STJ11104884)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

$174.83 - $775.58

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Immunocytochemistry analysis of PLK1 (green) in Hela using PLK1 antibody, and DAPI (blue)

Anti-PLK1 antibody (455-474 aa) [R07-3E0] (STJA0012682)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP

Reactivity :

Human/Rat

$357.08

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Western blot analysis of extracts from NIH-3T3 cells, using Plk1 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-PLK1 antibody (80-160 aa) (STJ96419)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

$64.80 - $340.20

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Anti-PLK1 antibody [3H10] (STJA0019789)

Clonality :

Monoclonal

Applications :

ELISA

Reactivity :

Human

$461.03 - $1,954.13

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Western blot analysis of 1) PC12 Cell, 2) HepG2 Cell lysate using PLK1 mouse monoclonal antibody diluted at 1:2,000.

Anti-PLK1 antibody [Mix] (STJ196690)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/WB

Reactivity :

Human/Mouse/Rat

$234.90 - $340.20

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Anti-PLK4 antibody (300-350) (STJ506393)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

$577.13

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