• Immunofluorescence analysis of U2OS cells using PCM1 Rabbit polyclonal antibody (STJ119061) at dilution of 1:200 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of Mouse brain, using PCM1 Rabbit polyclonal antibody (STJ119061) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.

Anti-PCM1 antibody (1-260) (STJ119061)

SKU:
STJ119061

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-PCM1 (1-260) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:5000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: PCM1
Gene ID: 5108
Uniprot ID: PCM1_HUMAN
Immunogen Region: 1-260
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-260 of human PCM1 (NP_006188.4).
Immunogen Sequence: MATGGGPFEDGMNDQDLPNW SNENVDDRLNNMDWGAQQKK ANRSSEKNKKKFGVESDKRV TNDISPESSPGVGRRRTKTP HTFPHSRYMSQMSVPEQAEL EKLKQRINFSDLDQRSIGSD SQGRATAANNKRQLSENRKP FNFLPMQINTNKSKDASTNP PNRETIGSAQCKELFASALS NDLLQNCQVSEEDGRGEPAM ESSQIVSRLVQIRDYITKAS SMREDLVEKNERSANVERL
Tissue Specificity Expressed in blood, bone marrow, breast, lymph node, ovary and thyroid.
Post Translational Modifications Ubiquitinated. Undergoes monoubiquitination catalyzed by the E3 ubiquitin-protein ligase MIB1 in proliferating cells, preventing cilia formation. Monoubiquitination by MIB1 is inhibited in response to cellular stress, such as ultraviolet light (UV) radiation or heat shock, resulting in cilia formation initiation. Variant Ser-159 is phosphorylated. Phosphorylated on multiple serine and threonine residues by DYRK3 during the G2-to-M transition, after the nuclear-envelope breakdown. Phosphorylation by DYRK3 promotes disassembly of pericentriolar material. Phosphorylation at Ser-372 mediated by PLK4 is required to maintain the integrity of centriolar satellites.
Function Required for centrosome assembly and function. Essential for the correct localization of several centrosomal proteins including CEP250, CETN3, PCNT and NEK2. Required to anchor microtubules to the centrosome. Also involved in cilium biogenesis by recruiting the BBSome, a ciliary protein complex involved in cilium biogenesis, to the centriolar satellites. Recruits the tubulin polyglutamylase complex (TPGC) to centriolar satellites.
Protein Name Pericentriolar Material 1 Protein
Pcm-1
Hpcm-1
Database Links Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-8854518
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Cytoplasmic Granule
Centriolar Satellite
Cilium Basal Body
Recruitment To The Centrosome Requires Microtubules And Dynein
The Majority Of The Protein Dissociates From The Centrosome During Metaphase And Subsequently Localizes To The Cleavage Site In Telophase
Displaced From Centriolar Satellites And Centrosome In Response To Cellular Stress
Such As Ultraviolet Light (Uv) Radiation Or Heat Shock
In A Process That Requires P38 Map Kinase Signaling
Alternative Antibody Names Anti-Pericentriolar Material 1 Protein antibody
Anti-Pcm-1 antibody
Anti-Hpcm-1 antibody
Anti-PCM1 antibody

Information sourced from Uniprot.org

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