• Immunohistochemistry analysis of paraffin-embedded human breast cancer using NDE1 antibody (STJ29192) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat spleen using NDE1 antibody (STJ29192) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Immunohistochemistry analysis of paraffin-embedded rat brain using NDE1 antibody (STJ29192) at dilution of 1:100 (40x lens). Perform microwave antigen retrieval with 10 mM PBS buffer pH 7. 2 before commencing with immunohistochemistry staining protocol.
  • Western blot analysis of extracts of various cell lines, using NDE1 antibody (STJ29192) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-NDE1 antibody (1-110) (STJ29192)

SKU:
STJ29192

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Host: Rabbit
Applications: WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NDE1 (1-110) is suitable for use in Western Blot and Immunohistochemistry research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IHC-P 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NDE1
Gene ID: 54820
Uniprot ID: NDE1_HUMAN
Immunogen Region: 1-110
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-110 of human NDE1 (NP_060138.1).
Immunogen Sequence: MEDSGKTFSSEEEEANYWKD LAMTYKQRAENTQEELREFQ EGSREYEAELETQLQQIETR NRDLLSENNRLRMELETIKE KFEVQHSEGYRQISALEDDL AQTKAIKDQL
Tissue Specificity Expressed in the neuroepithelium throughout the developing brain, including the cerebral cortex and cerebellum.
Post Translational Modifications Phosphorylated in mitosis. Phosphorylated in vitro by CDC2. Phosphorylation at Thr-246 is essential for the G2/M transition.
Function Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a post-mitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex.
Protein Name Nuclear Distribution Protein Nude Homolog 1
Nude
Database Links Reactome: R-HSA-141444
Reactome: R-HSA-2467813
Reactome: R-HSA-2500257
Reactome: R-HSA-2565942
Reactome: R-HSA-380259
Reactome: R-HSA-380270
Reactome: R-HSA-380284
Reactome: R-HSA-380320
Reactome: R-HSA-5620912
Reactome: R-HSA-5663220
Reactome: R-HSA-68877
Reactome: R-HSA-8854518
Reactome: R-HSA-9648025
Cellular Localisation Cytoplasm
Cytoskeleton
Microtubule Organizing Center
Centrosome
Chromosome
Centromere
Kinetochore
Spindle
Cleavage Furrow
Localizes To The Interphase And S Phase Centrosome
During Mitosis
Partially Associated With The Mitotic Spindle
Concentrates At The Plus Ends Of Microtubules Coincident With Kinetochores In Metaphase And Anaphase In A Cenpf-Dependent Manner
Also Localizes To The Cleavage Furrow During Cytokinesis
Manner
Alternative Antibody Names Anti-Nuclear Distribution Protein Nude Homolog 1 antibody
Anti-Nude antibody
Anti-NDE1 antibody
Anti-NUDE antibody

Information sourced from Uniprot.org

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