Anti-NPHP4 antibody (877-926) (STJ94402)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenThe antiserum was produced against synthesized peptide derived from human NPHP4. AA range:877-926
General Information
| Short Description | Rabbit polyclonal anti-Nephrocystin-4 (877-926) for use in IHC, IF and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/IF/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | IHC 1:100-1:300ELISA 1:40000IF 1:50-200 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20ยฐC for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NPHP4 |
| Gene ID | 261734 |
| Uniprot ID | NPHP4_HUMAN |
| Immunogen | The antiserum was produced against synthesized peptide derived from human NPHP4. AA range:877-926 |
| Immunogen Region | 877-926 |
| Specificity | Nephrocystin-4 Polyclonal Antibody detects endogenous levels of Nephrocystin-4 protein. |
Additional Info
| Tissue Specificity | Expressed in kidney, skeletal muscle, heart and liver, and to a lesser extent in brain and lung. |
| Function | Involved in the organization of apical junctions.the function is proposed to implicate a NPHP1-4-8 module. Does not seem to be strictly required for ciliogenesis. Required for building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells. Seems to recruit INT to basal bodies of motile cilia which subsequently interacts with actin-modifying proteins such as DAAM1. In cooperation with INVS may down-regulate the canonical Wnt pathway and promote the Wnt-PCP pathway by regulating expression and subcellular location of disheveled proteins. Stabilizes protein levels of JADE1 and promotes its translocation to the nucleus leading to cooperative inhibition of canonical Wnt signaling. Acts as a negative regulator of the hippo pathway by association with LATS1 and modifying LATS1-dependent phosphorylation and localization of WWTR1/TAZ. |
| Protein Name | Nephrocystin-4Nephroretinin |
| Database Links | Reactome: R-HSA-2028269Reactome: R-HSA-5620912 |
| Cellular Localisation | CytoplasmCytoskeletonCilium Basal BodyMicrotubule Organizing CenterCentrosomeCell JunctionTight JunctionNucleusIn Cultured Renal CellsIt Localizes Diffusely In The Cytoplasm ButAs Cells Approach ConfluenceIt Accumulates To Basolateral Tight JunctionsLocalizes To The Ciliary Transition ZoneIn The Retinal Photoreceptor Cell LayerLocalizes At The Connecting Cilium |
| Alternative Antibody Names | Anti-Nephrocystin-4 antibodyAnti-Nephroretinin antibodyAnti-NPHP4 antibodyAnti-KIAA0673 antibody |
Information sourced from Uniprot.org