• Western blot analysis of extracts of various cell lines, using NPHP4 antibody (STJ116333) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 30s.
  • Immunofluorescence analysis of H9C2 cells using NPHP4 rabbit polyclonal antibody (STJ116333) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using NPHP4 rabbit polyclonal antibody (STJ116333) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using NPHP4 rabbit polyclonal antibody (STJ116333) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Anti-NPHP4 antibody (1247-1426) (STJ116333)

SKU:
STJ116333

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-NPHP4 (1247-1426) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.01% Thimerosal, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:1000-1:3000
IF 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: NPHP4
Gene ID: 261734
Uniprot ID: NPHP4_HUMAN
Immunogen Region: 1247-1426
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1247-1426 of human NPHP4 (NP_055917.1).
Immunogen Sequence: RLSLVLRGTQTVRKVRAFTS HPQELKTDPKGVFVLPPRGV QDLHVGVRPLRAGSRFVHLN LVDVDCHQLVASWLVCLCCR QPLISKAFEIMLAAGEGKGV NKRITYTNPYPSRRTFHLHS DHPELLRFREDSFQVGGGET YTIGLQFAPSQRVGEEEILI YINDHEDKNEEAFCVKVIYQ
Tissue Specificity Expressed in kidney, skeletal muscle, heart and liver, and to a lesser extent in brain and lung.
Function Involved in the organization of apical junctions.the function is proposed to implicate a NPHP1-4-8 module. Does not seem to be strictly required for ciliogenesis. Required for building functional cilia. Involved in the organization of the subapical actin network in multiciliated epithelial cells. Seems to recruit INT to basal bodies of motile cilia which subsequently interacts with actin-modifying proteins such as DAAM1. In cooperation with INVS may down-regulate the canonical Wnt pathway and promote the Wnt-PCP pathway by regulating expression and subcellular location of disheveled proteins. Stabilizes protein levels of JADE1 and promotes its translocation to the nucleus leading to cooperative inhibition of canonical Wnt signaling. Acts as negative regulator of the hippo pathway by association with LATS1 and modifying LATS1-dependent phosphorylation and localization of WWTR1/TAZ.
Protein Name Nephrocystin-4
Nephroretinin
Database Links Reactome: R-HSA-2028269
Reactome: R-HSA-5620912
Cellular Localisation Cytoplasm
Cytoskeleton
Cilium Basal Body
Microtubule Organizing Center
Centrosome
Cell Junction
Tight Junction
Nucleus
In Cultured Renal Cells
It Localizes Diffusely In The Cytoplasm But
As Cells Approach Confluence
It Accumulates To Basolateral Tight Junctions
Localizes To The Ciliary Transition Zone
In The Retinal Photoreceptor Cell Layer
Localizes At The Connecting Cilium
Alternative Antibody Names Anti-Nephrocystin-4 antibody
Anti-Nephroretinin antibody
Anti-NPHP4 antibody
Anti-KIAA0673 antibody

Information sourced from Uniprot.org

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