| Host: |
Rabbit |
| Applications: |
WB/ELISA |
| Reactivity: |
Human/Mouse |
| Note: |
STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
| Short Description: |
Rabbit polyclonal antibody anti-Nephrocystin-1 (510-590 aa) is suitable for use in Western Blot and ELISA research applications. |
| Clonality: |
Polyclonal |
| Conjugation: |
Unconjugated |
| Isotype: |
IgG |
| Formulation: |
Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Purification: |
The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Concentration: |
1 mg/mL |
| Dilution Range: |
WB 1:500-2000ELISA 1:5000-20000 |
| Storage Instruction: |
Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
| Gene Symbol: |
NPHP1 |
| Gene ID: |
4867 |
| Uniprot ID: |
NPHP1_HUMAN |
| Immunogen Region: |
510-590 aa |
| Specificity: |
NPHP1 Polyclonal Antibody detects endogenous levels of protein. |
| Immunogen: |
Synthesized peptide derived from the human protein at the amino acid range 510-590 |
| Post Translational Modifications | Phosphorylation by CK2 is required for the interaction with PACS1 and the targeting to the base region of cilia. |
| Function | Together with BCAR1 it may play a role in the control of epithelial cell polarity. Involved in the organization of apical junctions in kidney cells together with NPHP4 and RPGRIP1L/NPHP8. Does not seem to be strictly required for ciliogenesis. Seems to help to recruit PTK2B/PYK2 to cell matrix adhesions, thereby initiating phosphorylation of PTK2B/PYK2 and PTK2B/PYK2-dependent signaling. May play a role in the regulation of intraflagellar transport (IFT) during cilia assembly. Required for normal retina development. In connecting photoreceptor cilia influences the movement of some IFT proteins such as IFT88 and WDR19. Involved in spermatogenesis. |
| Protein Name | Nephrocystin-1Juvenile Nephronophthisis 1 Protein |
| Database Links | Reactome: R-HSA-5620912 |
| Cellular Localisation | Cell JunctionAdherens JunctionCell ProjectionCiliumCytoplasmCytoskeletonCilium AxonemeTight JunctionIn The Retinal Photoreceptor Cell LayerLocalizes At The Connecting CiliumColocalizes With E-Cadherin And Bcar1 At Or Near The Cell-Cell Adherens JunctionsLocalized To Respiratory Cilia AxonemeLocalized To The Transition Zone Of Respiratory CiliaLocalized To The Transition Zone Of Photoreceptor-Connecting Cilia And Renal MonociliaIn Cultured Renal CellsIt Localizes Diffusely In The Cytoplasm ButAs Cells Approach ConfluenceIt Accumulates At Basolateral Tight Junctions |
| Alternative Antibody Names | Anti-Nephrocystin-1 antibodyAnti-Juvenile Nephronophthisis 1 Protein antibodyAnti-NPHP1 antibodyAnti-NPH1 antibody |
Information sourced from Uniprot.org
12 months for antibodies. 6 months for ELISA Kits. Please see website T&Cs for further guidance
