Anti-NDE1 antibody (270-350 aa) (STJ191089)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from the human protein at the amino acid range 270-350
General Information
| Short Description | Rabbit polyclonal anti-Nuclear distribution protein nudE homolog 1 (270-350 aa) for use in WB and ELISA in Human, Rat and Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/ELISA |
| Host | Rabbit |
| Reactivity | Human/Rat/Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000ELISA 1:5000-20000 |
| Formulation | Liquid in PBS containing 50% Glycerol and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | NDE1 |
| Gene ID | 54820 |
| Uniprot ID | NDE1_HUMAN |
| Immunogen | Synthesized peptide derived from the human protein at the amino acid range 270-350 |
| Immunogen Region | 270-350 aa |
| Specificity | NDE1 Polyclonal Antibody detects endogenous levels of protein. |
Additional Info
| Post Translational Modifications | Phosphorylated in mitosis. Phosphorylated in vitro by CDC2. Phosphorylation at Thr-246 is essential for the G2/M transition. |
| Function | Required for centrosome duplication and formation and function of the mitotic spindle. Essential for the development of the cerebral cortex. May regulate the production of neurons by controlling the orientation of the mitotic spindle during division of cortical neuronal progenitors of the proliferative ventricular zone of the brain. Orientation of the division plane perpendicular to the layers of the cortex gives rise to two proliferative neuronal progenitors whereas parallel orientation of the division plane yields one proliferative neuronal progenitor and a postmitotic neuron. A premature shift towards a neuronal fate within the progenitor population may result in an overall reduction in the final number of neurons and an increase in the number of neurons in the deeper layers of the cortex. Acts as a RAB9A/B effector that tethers RAB9-associated late endosomes to the dynein motor for their retrograde transport to the trans-Golgi network. |
| Protein Name | Nuclear Distribution Protein Nude Homolog 1Nude |
| Database Links | Reactome: R-HSA-141444Reactome: R-HSA-2467813Reactome: R-HSA-2500257Reactome: R-HSA-2565942Reactome: R-HSA-380259Reactome: R-HSA-380270Reactome: R-HSA-380284Reactome: R-HSA-380320Reactome: R-HSA-5620912Reactome: R-HSA-5663220Reactome: R-HSA-68877Reactome: R-HSA-8854518Reactome: R-HSA-9648025 |
| Cellular Localisation | CytoplasmCytoskeletonMicrotubule Organizing CenterCentrosomeChromosomeCentromereKinetochoreSpindleCleavage FurrowCytoplasmic Vesicle MembraneLocalizes To The Interphase And S Phase CentrosomeDuring MitosisPartially Associated With The Mitotic SpindleConcentrates At The Plus Ends Of Microtubules Coincident With Kinetochores In Metaphase And Anaphase In A Cenpf-Dependent MannerAlso Localizes To The Cleavage Furrow During CytokinesisMannerColocalizes With Rab9a To Membrane Vesicles |
| Alternative Antibody Names | Anti-Nuclear Distribution Protein Nude Homolog 1 antibodyAnti-Nude antibodyAnti-NDE1 antibodyAnti-NUDE antibody |
Information sourced from Uniprot.org