Metabolism | Research Area | St John's Laboratory

Western blot analysis of extracts of various cell lines, using GALE antibody (STJ28678) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-GALE antibody (129-348) (STJ28678)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Western blot analysis of various lysates using GALK1 Rabbit polyclonal antibody (STJ117469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 60S.

Anti-GALK1 antibody (1-120) (STJ117469)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£110.50 - £505.50

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Western blot analysis of various cells using GALK1 Polyclonal Antibody diluted at 1：1000

Anti-GALK1 antibody (1-50 aa) (STJ93207)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of lysates from K562 cells, primary antibody was diluted at 1:1000, 4°C over night

Anti-GALM antibody (40-120 aa) (STJ190534)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using GALNS Rabbit polyclonal antibody (STJ11105538) at 1:2000 dilution. Secondary antibody:HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection:ECL Basic Kit. Exposuretime: 45s.

Anti-GALNS antibody (27-307) (STJ11105538)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Mouse/Rat

£110.50 - £505.50

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Immunofluorescence analysis of L929 cells using GALNS Rabbit polyclonal antibody (STJ27414) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.

Anti-GALNS antibody (353-522) (STJ27414)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-GALNS antibody (STJA0008630)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of extracts of various cell lines, using GALT antibody (STJ11104139) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GALT antibody (1-100) [S4139RM] (STJ11104139)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£118.50 - £521.50

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Western blot analysis of various lysates using GALT Rabbit polyclonal antibody (STJ11104337) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-GALT antibody (1-379) (STJ11104337)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-GALT antibody (STJA0007665)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Anti-GAMT antibody (1-236) (STJ28680)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£110.50 - £505.50

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Anti-GAMT antibody (STJA0007328)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse/Rat

£49.00 - £261.00

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Western blot analysis of various lysates using GAPDH Rabbit pAb (STJ11103681) at 1:30000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 3s.

Anti-GAPDH antibody (1-335) (STJ11103681)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP

Reactivity :

Human/Mouse/Rat

£132.50 - £241.50

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Western blot analysis of various lysates using GAPDH Rabbit pAb (STJ11103691) at 1:10000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-GAPDH antibody (1-335) (STJ11103691)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/IP

Reactivity :

Human/Mouse/Rat

£132.50 - £241.50

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Western blot analysis of various lysates using GAPDH Mouse mAb (STJ11103682) at 1:10000 dilution incubated overnight at 4°C. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 1s.

Anti-GAPDH antibody (1-335) [S2MR] (STJ11103682)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£132.50 - £241.50

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Western blot analysis of various lysates, using GAPDH Mouse mAb (High Dilution) (STJ11103693) at 1:640000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (STJS000854) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 30s.

Anti-GAPDH antibody (1-335) [S3MM] (STJ11103693)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Western blot analysis of various lysates, using HRP-conjugated GAPDH Mouse mAb (STJ11105762) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Basic Kit <br/>Exposure time: 10s.

Anti-GAPDH antibody (1-335) [S5762MM] {HRP} (STJ11105762)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£201.50 - £379.50

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Immunofluorescence analysis of Hela cell. 1, Cyclin D1 Polyclonal Antibody (red) was diluted at 1:200 (4°C overnight). GAPDH monoclonal antibody (2B8) (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 594 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). Goat Anti Mouse Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min).

Anti-GAPDH antibody (200-300 aa) [2B8] (STJ96931)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Pig/Sheep/Insect/Yeast/Cow

£49.00 - £261.00

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Anti-GAPDH antibody (240 aa C-Term) {DyLight®405} (STJ140264)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IF

Reactivity :

Human/Rat/Mouse/Zebrafish/Canine/Monkey

£141.50 - £426.50

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Anti-GAPDH antibody (240 aa C-Term) {DyLight®488} (STJ140265)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IF

Reactivity :

Human/Rat/Mouse/Zebrafish/Canine/Monkey

£141.50 - £426.50

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Anti-GAPDH antibody (240 aa C-Term) {DyLight®550} (STJ140266)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IF

Reactivity :

Human/Rat/Mouse/Zebrafish/Canine/Monkey

£141.50 - £426.50

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Anti-GAPDH antibody (240 aa C-Term) {DyLight®633} (STJ140267)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IF

Reactivity :

Human/Rat/Mouse/Zebrafish/Canine/Monkey

£141.50 - £426.50

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Anti-GAPDH antibody at 1:2500 dilution; lysates at 50 µg per lane; Rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-GAPDH antibody (240aa C-Term) (STJ140037)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Zebrafish/Canine

£86.50 - £534.50

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Anti-GADPH antibody at 1:1000 dilution; lysates at 100 µg per lane; rabbit polyclonal to goat IgG (HRP) at 1:10000 dilution

Anti-GAPDH antibody (240aa C-Term) (STJ140038)

Clonality :

Polyclonal

Host :

Goat

Applications :

WB/IHC-F/IHC-P/IF

Reactivity :

Human/Rat/Mouse/Monkey/Canine

£101.50 - £394.50

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Anti-GAPDH antibody (250-300) (STJ506149)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£427.50

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Anti-GAPDH antibody (250-300) {Biotin} (STJ506183)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Anti-GAPDH antibody (250-300) {FITC} (STJ506184)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IP/WB

Reactivity :

Human

£528.50

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Anti-GAPDH antibody (280-333) (STJ13100138)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Rat/Mouse/Human/Marmoset

£331.50

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Western blot analysis of various lysates, using GAPDH antibody ( STJ11101594) at 1:100000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-GAPDH antibody (4-335) [S4MR] (STJ11101594)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£0.00

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Anti-GAPDH antibody (C-Term) (STJ13100140)

Clonality :

Polyclonal

Host :

Sheep

Applications :

WB/IHC

Reactivity :

Rat/Mouse/Human

£331.50

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WB on human neutrophil lysate using Sheep antibody to c-terminal of GAPDH (STJ13100141) at a concentration of 20 ug/ml.

Anti-GAPDH antibody (C-Term) (STJ13100141)

Clonality :

Polyclonal

Host :

Sheep

Applications :

IHC/WB

Reactivity :

Rat/Mouse/Human

£331.50

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Anti-GAPDH antibody (Human Spec) [SRM15102] (STJ11109832)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£0.00

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Anti-GAPDH antibody (Met1-Glu335) [1A200] (STJA0022029)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£174.50 - £250.50

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Anti-GAPDH antibody (Met1-Glu335) [1A201] (STJA0022030)

Clonality :

Monoclonal

Host :

Mouse

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£174.50 - £250.50

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WB on rat and mouse tissue lysates. Lysates were prepared in Ripa buffer containing 1% Triton X-100, 1% SDS and 1% SDC. Blocking: 1% LFDM for 30 min at RT; primary antibody dilution 1: 30000 incubated at 4C overnight.

Anti-GAPDH antibody (STJ13100139)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IHC/WB

Reactivity :

Rat/Mouse/Human/Marmoset

£331.50

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Anti-GAPDH antibody (STJ13100504)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Rat/Mouse/Human/Marmoset

£331.50

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Loss of NDUFS1 promotes gastric cancer progression by activating the mitochondrial ROS-HIF1α-FBLN5 signaling pathway. BRITISH JOURNAL OF CANCER Jin Zhou WB Human 1:5000 MKN45 cell, N87 cell, GES-1 cell, AGS cell, HGC-27 cell, KATO3 cell, SNU-1 cell

Anti-GAPDH antibody (STJ197380)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF

Reactivity :

Human/Mouse/Rat/Rabbit/Monkey/Sheep/Fish/Chicken/Guinea Pig/Duck

£49.00 - £261.00

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Western blot analysis of Zebrafish skeletal muscle, diluted at 1:5000. Secondary antibody (STJ99666) was diluted at 1:20000

Anti-GAPDH antibody (STJA0006704)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Zebrafish

£49.00 - £261.00

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Anti-GAPDH antibody [2B8] {AbFluor™ 350} (STJA0006053)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Pig/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-GAPDH antibody [2B8] {AbFluor™ 405} (STJA0006054)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Pig/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-GAPDH antibody [2B8] {AbFluor™ 488} (STJA0006055)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Pig/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-GAPDH antibody [2B8] {AbFluor™ 555} (STJA0006056)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Pig/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-GAPDH antibody [2B8] {AbFluor™ 594} (STJA0006057)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Pig/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-GAPDH antibody [2B8] {AbFluor™ 647} (STJA0006058)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Pig/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-GAPDH antibody [2B8] {AbFluor™ 680} (STJA0006059)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Pig/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-GAPDH antibody [2B8] {Biotin} (STJA0006060)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Pig/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-GAPDH antibody [2B8] {Cy3} (STJA0006061)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Pig/Sheep/Insect/Yeast

£149.00 - £248.00

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Anti-GAPDH antibody [2B8] {Cy5} (STJA0006062)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IF/IHC

Reactivity :

Human/Rat/Mouse/Monkey/Dog/Chicken/Hamster/Rabbit/Pig/Sheep/Insect/Yeast

£149.00 - £248.00

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