• Immunofluorescence analysis of HeLa cells using ECI1 Rabbit polyclonal antibody (STJ23465). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.
  • Immunohistochemistry analysis of ECI1 in paraffin-embedded human colon carcinoma tissue using ECI1 Rabbit polyclonal antibody (STJ23465) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0. 01 M citrate buffer (pH 6. 0) prior to immunohistochemistry staining.
  • Western blot analysis of various lysates using ECI1 Rabbit polyclonal antibody (STJ23465) at 1:2000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/ proteins: 25 Mu g per lane. Blocking buffer: 3 % nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 45s.

Anti-ECI1 antibody (63-302) (STJ23465)

SKU:
STJ23465

£169.50 - £449.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ECI1 (63-302) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ECI1
Gene ID: 1632
Uniprot ID: ECI1_HUMAN
Immunogen Region: 63-302
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 63-302 of human ECI1 (NP_001910.2).
Immunogen Sequence: KNPPVNSLSLEFLTELVISL EKLENDKSFRGVILTSDRPG VFSAGLDLTEMCGRSPAHYA GYWKAVQELWLRLYQSNLVL VSAINGACPAGGCLVALTCD YRILADNPRYCIGLNETQLG IIAPFWLKDTLENTIGHRAA ERALQLGLLFPPAEALQVGI VDQVVPEEQVQSTALSAIAQ WMAIPDHARQLTKAMMRKAT ASRLVTQRDADVQNFVSFIS KDSIQKSLQMYLERLKEEK
Tissue Specificity Expressed in liver (at protein level).
Function Able to isomerize both 3-cis and 3-trans double bonds into the 2-trans form in a range of enoyl-CoA species.
Protein Name Enoyl-Coa Delta Isomerase 1 - Mitochondrial
3 -2-Trans-Enoyl-Coa Isomerase
Delta(3 -Delta(2-Enoyl-Coa Isomerase
D3 -D2-Enoyl-Coa Isomerase
Dodecenoyl-Coa Isomerase
Database Links Reactome: R-HSA-77288
Cellular Localisation Mitochondrion Matrix
Alternative Antibody Names Anti-Enoyl-Coa Delta Isomerase 1 - Mitochondrial antibody
Anti-3 -2-Trans-Enoyl-Coa Isomerase antibody
Anti-Delta(3 -Delta(2-Enoyl-Coa Isomerase antibody
Anti-D3 -D2-Enoyl-Coa Isomerase antibody
Anti-Dodecenoyl-Coa Isomerase antibody
Anti-ECI1 antibody
Anti-DCI antibody

Information sourced from Uniprot.org

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