• Immunofluorescence analysis of U-2 OS cells using DTYMK Rabbit polyclonal antibody (STJ28453) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using DTYMK Rabbit polyclonal antibody (STJ28453) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
  • Western blot analysis of extracts of various cell lines, using DTYMK antibody (STJ28453) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-DTYMK antibody (1-212) (STJ28453)

SKU:
STJ28453

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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-DTYMK (1-212) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: DTYMK
Gene ID: 1841
Uniprot ID: KTHY_HUMAN
Immunogen Region: 1-212
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 1-212 of human DTYMK (NP_036277.2).
Immunogen Sequence: MAARRGALIVLEGVDRAGKS TQSRKLVEALCAAGHRAELL RFPERSTEIGKLLSSYLQKK SDVEDHSVHLLFSANRWEQV PLIKEKLSQGVTLVVDRYAF SGVAFTGAKENFSLDWCKQP DVGLPKPDLVLFLQLQLADA AKRGAFGHERYENGAFQERA LRCFHQLMKDTTLNWKMVDA SKSIEAVHEDIRVLSEDAIR TATEKPLGELWK
Function Catalyzes the phosphorylation of thymidine monophosphate (dTMP) to thymidine diphosphate (dTDP), the immediate precursor for the DNA building block dTTP, with ATP as the preferred phosphoryl donor in the presence of Mg(2+).
Protein Name Thymidylate Kinase
Dtmp Kinase
Database Links Reactome: R-HSA-499943
Alternative Antibody Names Anti-Thymidylate Kinase antibody
Anti-Dtmp Kinase antibody
Anti-DTYMK antibody
Anti-CDC8 antibody
Anti-TMPK antibody
Anti-TYMK antibody

Information sourced from Uniprot.org

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