• Western blot analysis of extracts of HepG2 cells, using ECSIT antibody (STJ110114) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
  • Immunofluorescence analysis of H9C2 cells using ECSIT rabbit polyclonal antibody (STJ110114) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of L929 cells using ECSIT rabbit polyclonal antibody (STJ110114) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.
  • Immunofluorescence analysis of U2OS cells using ECSIT rabbit polyclonal antibody (STJ110114) at dilution of 100 (40x lens). Blue: DAPI for nuclear staining.

Anti-ECSIT antibody (50-296) (STJ110114)

SKU:
STJ110114

£112.50 - £522.50
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Host: Rabbit
Applications: WB/IF
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Rabbit polyclonal antibody anti-ECSIT (50-296) is suitable for use in Western Blot and Immunofluorescence research applications.
Clonality: Polyclonal
Conjugation: Unconjugated
Isotype: IgG
Formulation: PBS with 0.02% Sodium Azide, 50% Glycerol, pH7.3.
Purification: Affinity purification
Dilution Range: WB 1:500-1:2000
IF/ICC 1:50-1:200
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: ECSIT
Gene ID: 51295
Uniprot ID: ECSIT_HUMAN
Immunogen Region: 50-296
Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 50-296 of human ECSIT (NP_001135936.1).
Immunogen Sequence: SEQSLVPSPPEPRQRPTKAL VPFEDLFGQAPGGERDKASF LQTVQKFAEHSVRKRGHIDF IYLALRKMREYGVERDLAVY NQLLNIFPKEVFRPRNIIQR IFVHYPRQQECGIAVLEQME NHGVMPNKETEFLLIQIFGR KSYPMLKLVRLKLWFPRFMN VNPFPVPRDLPQDPVELAMF GLRHMEPDLSARVTIYQVPL PKDSTGAADPPQPHIVGSGR DAGGVEPLLPDAAGPGVCE
Post Translational Modifications Ubiquitinated on Lys-372.leading to translocation in the nucleus together with RELA and NFKB1 and expression of NF-kappa-B-dependent genes.
Function Adapter protein that plays a role in different signaling pathways including TLRs and IL-1 pathways or innate antiviral induction signaling. Plays a role in the activation of NF-kappa-B by forming a signal complex with TRAF6 and TAK1/MAP3K7 to activate TAK1/MAP3K7 leading to activation of IKKs. Once ubiquitinated, interacts with the dissociated RELA and NFKB1 proteins and translocates to the nucleus where it induces NF-kappa-B-dependent gene expression. Plays a role in innate antiviral immune response by bridging the pattern recognition receptors RIGI and MDA5/IFIT1 to the MAVS complex at the mitochondrion. Promotes proteolytic activation of MAP3K1. Involved in the BMP signaling pathway. Required for normal embryonic development. As part of the MCIA complex, involved in the assembly of the mitochondrial complex I.
Protein Name Evolutionarily Conserved Signaling Intermediate In Toll Pathway - Mitochondrial
Protein Sitpec
Database Links Reactome: R-HSA-166058
Reactome: R-HSA-6799198
Reactome: R-HSA-975138
Reactome: R-HSA-975871
Cellular Localisation Cytoplasm
Nucleus
Mitochondrion
Alternative Antibody Names Anti-Evolutionarily Conserved Signaling Intermediate In Toll Pathway - Mitochondrial antibody
Anti-Protein Sitpec antibody
Anti-ECSIT antibody

Information sourced from Uniprot.org

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