Anti-ECHS1 antibody (STJA0006828)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenSynthesized peptide derived from human ECHS1
General Information
| Short Description | Rabbit polyclonal anti-Enoyl-CoA hydratase, mitochondrial for use in WB in Human, Mouse and Rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | 1 mg/mL |
| Purification | The antibody was affinity-purified from rabbit antiserum by affinity-chromatography using epitope-specific immunogen. |
| Dilution Range | WB 1:500-2000 |
| Formulation | Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | ECHS1 |
| Gene ID | 1892 |
| Uniprot ID | ECHM_HUMAN |
| Immunogen | Synthesized peptide derived from human ECHS1 |
| Specificity | This antibody detects endogenous levels of ECHS1 at Human, Mouse, Rat |
Additional Info
| Function | Converts unsaturated trans-2-enoyl-CoA species ((2E)-enoyl-CoA) to the corresponding (3S)-3hydroxyacyl-CoA species through addition of a water molecule to the double bond. Catalyzes the hydration of medium- and short-chained fatty enoyl-CoA thioesters from 4 carbons long (C4) up to C16. Has high substrate specificity for crotonyl-CoA ((2E)-butenoyl-CoA) and moderate specificity for acryloyl-CoA, 3-methylcrotonyl-CoA (3-methyl-(2E)-butenoyl-CoA) and methacrylyl-CoA ((2E)-2-methylpropenoyl-CoA). Can bind tiglyl-CoA (2-methylcrotonoyl-CoA), but hydrates only a small amount of this substrate. Plays a key role in the beta-oxidation spiral of short- and medium-chain fatty acid oxidation. At a lower rate than the hydratase reaction, catalyzes the isomerase reaction of trans-3-enoyl-CoA species (such as (3E)-hexenoyl-CoA) to trans-2-enoyl-CoA species (such as (2E)-hexenoyl-CoA), which are subsequently hydrated to 3(S)-3-hydroxyacyl-CoA species (such as (3S)-hydroxyhexanoyl-CoA). |
| Protein Name | Enoyl-Coa Hydratase - MitochondrialMechMech1Enoyl-Coa Hydratase 1Echs1Short-Chain Enoyl-Coa HydrataseSceh |
| Database Links | Reactome: R-HSA-70895Reactome: R-HSA-77310Reactome: R-HSA-77346Reactome: R-HSA-77348Reactome: R-HSA-77350Reactome: R-HSA-77352Reactome: R-HSA-9916720 |
| Cellular Localisation | Mitochondrion Matrix |
| Alternative Antibody Names | Anti-Enoyl-Coa Hydratase - Mitochondrial antibodyAnti-Mech antibodyAnti-Mech1 antibodyAnti-Enoyl-Coa Hydratase 1 antibodyAnti-Echs1 antibodyAnti-Short-Chain Enoyl-Coa Hydratase antibodyAnti-Sceh antibodyAnti-ECHS1 antibody |
Information sourced from Uniprot.org