Western Blot Antibodies | St John's Laboratory

Immunohistochemistry analysis of CDKN1A/p21CIP1 in paraffin-embedded human breast cancer using CDKN1A/p21CIP1 Rabbit polyclonal antibody (STJ111165) at dilution of 1:100 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6. 0 before commencing with immunohistochemistry staining protocol.

Anti-CDKN1A antibody (1-164) (STJ111165)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IP/ELISA

Reactivity :

Human/Mouse

£112.50 - £524.50

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Western blot analysis of MCF7, using CDKN1A/p21CIP1 antibody (STJ23069) at 1:900 dilution. Secondary antibody: HRP Goat Anti-rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% non-fat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDKN1A antibody (1-164) (STJ23069)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Anti-CDKN1A antibody (1-164) [S1MR] (STJ11101631)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunofluorescence analysis of Hela cell. 1, p21 Polyclonal Antibody (green) was diluted at 1:200 (4°C overnight). 2, Goat Anti Rabbit Alexa Fluor 488 Catalog: (NA was diluted at 1:1000 (room temperature, 50min). 3 DAPI (blue) 10min.

Anti-CDKN1A antibody (111-160 aa) (STJ94862)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

IF/WB/IHC/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis of p21 in MCF-7 lysates using p21 antibody.

Anti-CDKN1A antibody (148-164 aa) [R07-3K5] (STJA0011727)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB

Reactivity :

Human/Mouse

£246.50

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Anti-CDKN1A antibody (Full Length) [M513] (STJA0005501)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ICC/IHC/IP

Reactivity :

Human

£319.50

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Anti-CDKN1A/p21 antibody [SRM] (STJ11106855)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse

£146.50 - £642.50

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Western blot analysis of lysates from PC-12 cells, using CDKN1B/p27KIP1 Rabbit pAb (STJ115573) at 1:1000 dilution. <br/>Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. <br/>Lysates/proteins: 25 Mu g per lane. <br/>Blocking buffer: 3% nonfat dry milk in TBST. <br/>Detection: ECL Enhanced Kit <br/>Exposure time: 90s.

Anti-CDKN1B antibody (1-198) (STJ115573)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of extracts of various cell lines, using CDKN1B/p27KIP1 antibody (STJ119057) at 1:900 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 20s.

Anti-CDKN1B antibody (1-198) (STJ119057)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Immunofluorescence analysis of U-2 OS cells using p27 KIP 1 antibody (STJ23072) at dilution of 1:100. Blue: DAPI for nuclear staining.

Anti-CDKN1B antibody (1-198) (STJ23072)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Whole cell lysates were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p27Kip1 (ABT-p27) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: MCF7Predicted band size: 22kDaObserved band size: 24kDa

Anti-CDKN1B antibody (100-198 aa) [ABT-p27] (STJ196998)

Clonality :

Monoclonal

Host :

Mouse

Applications :

IHC/IF/WB/ELISA

Reactivity :

Human

£174.00 - £252.00

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Immunohistochemical analysis of paraffin-embedded Human-liver-cancer tissue. 1, p27 Polyclonal Antibody was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.

Anti-CDKN1B antibody (149-198 aa) (STJ94866)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse/Cow

£48.00 - £252.00

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Western blot analysis using p27 monoclonal antibody against CDKN1B-hIgGFc transfected HEK293 cell lysate.

Anti-CDKN1B antibody [3D8] (STJ98298)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Western blot analysis of extracts of various cell lines, using CDKN1C/p57 Kip2 Rabbit monoclonal antibody (STJ11102330) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 10s.

Anti-CDKN1C antibody (1-100) [S0MR] (STJ11102330)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£129.50 - £574.50

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Western blot analysis of extracts of various cell lines, using CDKN1C/p57 Kip2 antibody (STJ23075) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 180s.

Anti-CDKN1C antibody (1-107) (STJ23075)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Western blot analysis of Jurkat cells using p57 Polyclonal Antibody.. Secondary antibody was diluted at 1:20000

Anti-CDKN1C antibody (241-290 aa) (STJ96707)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ELISA

Reactivity :

Human/Mouse/Rat

£48.00 - £252.00

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Western blot analysis using p57 monoclonal antibody against HEK293 (1) and CDKN1C-hIgGFc transfected HEK293 (2) cell lysate.

Anti-CDKN1C antibody [3E3] (STJ98302)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£144.00 - £348.00

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Anti-CDKN2A antibody (1-100 aa) [SRM] (STJ11107252)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human/Mouse/Rat

£146.50 - £642.50

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Immunoprecipitation analysis of 200 Mu g extracts of 293T cells, using 3 Mu g CDKN2A/p16INK4a antibody (STJ112923). Western blot was performed from the immunoprecipitate using CDKN2A/p16INK4a antibody (STJ112923) at a dilution of 1:1000.

Anti-CDKN2A antibody (1-156) (STJ112923)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IP/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of extracts of various cell lines, using CDKN2A/p16INK4a antibody (STJ113767) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.

Anti-CDKN2A antibody (1-156) (STJ113767)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£112.50 - £524.50

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Immunofluorescence analysis of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit polyclonal antibody (STJ23076) at dilution of 1:200 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Anti-CDKN2A antibody (1-156) (STJ23076)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IF/ICC/ELISA

Reactivity :

Human

£129.50 - £642.50

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Western blot analysis of various cells using p16 Polyclonal Antibody

Anti-CDKN2A antibody (10-59 aa) (STJ94858)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse

£48.00 - £252.00

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Whole cell lysates of Hela were separated by 12% SDS-PAGE, and the membrane was blotted with anti-p16 (PTR2301) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size: 16kDaObserved band size: 16kDa

Anti-CDKN2A antibody (100-156 aa) [PTR2301] (STJ196735)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Anti-CDKN2A antibody (100-156 aa) [PTR2302] (STJ196736)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC

Reactivity :

Human/Mouse/Rat

£174.00 - £252.00

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Confocal imaging of HeLa cells using [KO Validated] CDKN2A/p16INK4a Rabbit monoclonal antibody (STJ113253, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (dilution 1:500) (Red). The cells were counterstained with Alpha-Tubulin Mouse monoclonal antibody ( dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) antibody ( dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

Anti-CDKN2A antibody (57-156) [S3MR] (STJ113253)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IHC-P/IF/ICC/ELISA

Reactivity :

Human

£146.50 - £642.50

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Western blot analysis of various cells using p14 Polyclonal Antibody diluted at 1：500

Anti-CDKN2A antibody (71-120 aa) (STJ94855)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of CDKN2A/p14ARF in PC-3 lysates using CDKN2A antibody.

Anti-CDKN2A antibody (75-132 aa) [R01-1Q5] (STJA0015047)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF/IP/FC

Reactivity :

Human

£264.50

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Western blot analysis of 293T 22RV1 using p16 antibody. Antibody was diluted at 1:2000. Secondary antibody was diluted at 1:20000

Anti-CDKN2A antibody (90-170 aa) (STJ97617)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Anti-CDKN2A antibody (94-111) (STJ502022)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CDKN2A antibody (94-111) {Biotin} (STJ502027)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CDKN2A antibody (94-111) {FITC} (STJ502028)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CDKN2A antibody (Mid-Region) (STJ502021)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£427.50

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Anti-CDKN2A antibody (Mid-Region) {Biotin} (STJ502025)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Anti-CDKN2A antibody (Mid-Region) {FITC} (STJ502026)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/WB

Reactivity :

Human/Mouse/Rat

£528.50

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Immunohistochemistry of baboon lung with Anti-CDKN2A primary antibody (STJ502020) at 1:100 dilution in buffer. Section was treated with DAB and Haematoxylin stain.

Anti-CDKN2A antibody (N-Term) (STJ502020)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£427.50

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Anti-CDKN2A antibody (N-Term) {Biotin} (STJ502023)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Anti-CDKN2A antibody (N-Term) {FITC} (STJ502024)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

ELISA/IHC/IP/WB

Reactivity :

Human/Monkey/Mouse/Rat

£528.50

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Western blot analysis using p16 monoclonal antibody against truncated p16 recombinant protein.

Anti-CDKN2A antibody [1E12E10] (STJ98296)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC/IF/ELISA

Reactivity :

Human/Mouse/Rat

£144.00 - £348.00

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EB07091 (1µg/ml) staining of HeLa lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CDKN2A-Isoform 3 antibody (Internal) (STJ70854)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB

Reactivity :

Human

£312.50

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Western blot analysis of various lysates, using CDKN2A/p16INK4a Rabbit monoclonal antibody (STJ11105116) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3s.

Anti-CDKN2A/p16INK4a antibody (1-156 aa) [S5116RM] (STJ11105116)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human

£129.50 - £574.50

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Western blot analysis of CDKN2A/p16INK4a in 3T3, Hela lysates using CDKN2A antibody.

Anti-CDKN2A/p16INK4a antibody (Full-length) [R02-6H1] (STJA0011129)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/IP

Reactivity :

Human/Mouse

£264.50

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Immunocytochemistry analysis of CDKN2A/p16INK4a (green) in Hela using CDKN2A/p16INK4a antibody, and DAPI (blue)

Anti-CDKN2A/p16INK4a antibody (Full-length) [R04-8C9] (STJA0011127)

Clonality :

Monoclonal

Host :

Rabbit

Applications :

WB/ICC/IF

Reactivity :

Human/Mouse

£246.50

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Anti-CDKN2A/p16INK4a antibody [SM0438] (STJA0010438)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB

Reactivity :

Human

£170.50 - £233.50

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Immunohistochemistry analysis of paraffin-embedded Human cervix cancer using CDKN2A/p16INK4a Mouse mAb (STJ11106646) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0. 01M Tris/EDTA Buffer (pH 9. 0) prior to immunohistochemistry staining.

Anti-CDKN2A/p16INK4a antibody [SMM] (STJ11106646)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/IHC-P/ELISA

Reactivity :

Human

£146.50 - £642.50

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STJ70855 (1µg/ml) staining of HeLa Lysate (35µg protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

Anti-CDKN2A/p16INK4a-Isoform 1 antibody (C-Term) (STJ70855)

Clonality :

Polyclonal

Host :

Goat

Applications :

Pep-ELISA/WB/IHC

Reactivity :

Human

£312.50

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Anti-CDKN2AIP antibody (STJ192220)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Rat/Mouse

£48.00 - £252.00

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Western blot analysis of lysates from Rat lung using CDKN2B Rabbit polyclonal antibody (STJ27652) at 1:500 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (STJS000856) at 1:10000 dilution. Lysates/proteins: 25 Mu g per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.

Anti-CDKN2B antibody (1-138) (STJ27652)

Clonality :

Polyclonal

Host :

Rabbit

Applications :

WB/ELISA

Reactivity :

Human/Mouse/Rat

£112.50 - £524.50

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Whole cell lysates were separated by 15% SDS-PAGE, and the membrane was blotted with anti-p15 (PTR2192) antibody. The HRP-conjugated Goat anti- mouse IgG (H + L) antibody was used to detect the antibody. Lane 1: HelaPredicted band size:15kDaObserved band size: 15kDa

Anti-CDKN2B antibody (50-138) [PTR2192] (STJA0006247)

Clonality :

Monoclonal

Host :

Mouse

Applications :

WB/ELISA

Reactivity :

Human

£48.00 - £252.00

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