• Immunohistochemical analysis of paraffin-embedded Human Placenta Tissue using HP-1 Alpha Mouse mAb diluted at 1:200
  • Western blot analysis of 1) Hela Cell Lysate, 2) 3T3 Cell Lysate, 3) PC12 Cell Lysate using HP-1 Gamma Alpha Mouse mAb diluted at 1:1000.
  • Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1 Alpha Mouse mAb diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded Human Colon Carcinoma Tissue using HP-1 Alpha Mouse mAb diluted at 1:200
  • Immunohistochemical analysis of paraffin-embedded Rat-lung tissue. 1, HP-1 Alpha Mouse monoclonal antibody (5E3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunohistochemical analysis of paraffin-embedded Human-uterus tissue. 1, HP-1 Alpha Mouse monoclonal antibody (5E3) was diluted at 1:200 (4°C, overnight). 2, Sodium citrate pH 6.0 was used for antibody retrieval (>98°C, 20min). 3, Secondary antibody was diluted at 1:200 (room tempeRature, 30min). Negative control was used by secondary antibody only.
  • Immunofluorescence analysis of human-uterus tissue. 1, HP-1 Alpha Mouse monoclonal antibody (5E3) (red) was diluted at 1:200 (4°C, overnight). 2, Cy3 labled Secondary antibody was diluted at 1:300 (room temperature, 50min).3, Picture B: DAPI (blue) 10min. Picture A:Target. Picture B: DAPI. Picture C: merge of A+B

Anti-CBX5 antibody (STJ98895)

SKU:
STJ98895

£46.00 - £226.00
Current Stock:
Host: Mouse
Applications: IF/WB/IHC
Reactivity: Human/Mouse/Rat
Note: STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS.
Short Description: Mouse monoclonal antibody anti-Chromobox protein homolog 5 is suitable for use in Immunofluorescence, Western Blot and Immunohistochemistry research applications.
Clonality: Monoclonal
Conjugation: Unconjugated
Formulation: Liquid in PBS containing 50% Glycerol, 0.5% BSA and 0.02% Sodium Azide.
Purification: The antibody was affinity-purified from mouse antiserum by affinity-chromatography using epitope-specific immunogen.
Concentration: 1 mg/mL
Dilution Range: IF 1:50-200
WB 1:500-2000
IHC-P 1:50-300
Storage Instruction: Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles.
Gene Symbol: CBX5
Gene ID: 23468
Uniprot ID: CBX5_HUMAN
Specificity: The antibody detects endogenous HP-1 Alpha protein
Immunogen: Recombinant Protein of HP-1 Alpha
Function Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins.
Protein Name Chromobox Protein Homolog 5
Antigen P25
Heterochromatin Protein 1 Homolog Alpha
Hp1 Alpha
Database Links Reactome: R-HSA-4551638
Reactome: R-HSA-8953750
Reactome: R-HSA-983231
Cellular Localisation Nucleus
Chromosome
Centromere
Colocalizes With Hnrnpu In The Nucleus
Component Of Centromeric And Pericentromeric Heterochromatin
Associates With Chromosomes During Mitosis
Associates Specifically With Chromatin During Metaphase And Anaphase
Localizes To Sites Of Dna Damage
Alternative Antibody Names Anti-Chromobox Protein Homolog 5 antibody
Anti-Antigen P25 antibody
Anti-Heterochromatin Protein 1 Homolog Alpha antibody
Anti-Hp1 Alpha antibody
Anti-CBX5 antibody
Anti-HP1A antibody

Information sourced from Uniprot.org

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