Anti-CBX5 antibody (50-100 aa) (STJ13100980)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
ImmunogenA synthetic peptide from aa region 50-100 of mouse CBX5 conjugated to an immunogenic carrier protein was used as the antigen.
General Information
| Short Description | Rabbit polyclonal anti-CBX5 (50-100 aa) for use in IHC and WB in Mouse samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | IHC/WB |
| Host | Rabbit |
| Reactivity | Mouse |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Purification | IgG purified |
| Dilution Range | IHC, WB (confirmed by recombinant protein). A dilution of 1:300 to 1:2000 is recommended. The optimal dilution should be determined by the end user. Not yet tested in other applications. |
| Formulation | Lyophilised |
| Storage Instruction | Maintain the lyophilised/reconstituted antibodies frozen at-20°C for long term storage and refrigerated at 2-8°C for a shorter term. When reconstituting, Glycerol (1:1) may be added for an additional stability. Avoid freeze and thaw cycles. |
Target Information
| Gene Symbol | Cbx5 |
| Gene ID | 12419 |
| Uniprot ID | CBX5_MOUSE |
| Immunogen | A synthetic peptide from aa region 50-100 of mouse CBX5 conjugated to an immunogenic carrier protein was used as the antigen. |
| Immunogen Region | 50-100 aa |
| Specificity | Specific for CBX5. |
Additional Info
| Function | Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). May contribute to the association of heterochromatin with the inner nuclear membrane by interactions with the lamin-B receptor (LBR). Involved in the formation of kinetochore through interaction with the MIS12 complex subunit NSL1. Required for the formation of the inner centromere. Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). Can interact with lamin-B receptor (LBR). This interaction can contribute to the association of the heterochromatin with the inner nuclear membrane. Involved in the formation of functional kinetochore through interaction with MIS12 complex proteins. |
| Protein Name | Chromobox Protein Homolog 5Heterochromatin Protein 1 Homolog AlphaHp1 Alpha |
| Database Links | Reactome: R-MMU-983231 |
| Cellular Localisation | NucleusChromosomeCentromereColocalizes With Hnrnpu In The NucleusComponent Of Centromeric And Pericentromeric HeterochromatinAssociates With Chromosomes During MitosisAssociates Specifically With Chromatin During Metaphase And AnaphaseLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Chromobox Protein Homolog 5 antibodyAnti-Heterochromatin Protein 1 Homolog Alpha antibodyAnti-Hp1 Alpha antibodyAnti-Cbx5 antibodyAnti-Hp1a antibody |
Information sourced from Uniprot.org