Anti-CBX5 antibody (1-191) (STJ22922)
SPECIFICATIONS
ClonalityPolyclonal
HostRabbit
ConjugationUnconjugated
IsotypeIgG
General Information
| Short Description | Rabbit polyclonal CBX5 (1-191) antibody for use in WB, IHC-P, IF, ICC, IP, ELISA and ChIP in human, mouse and rat samples. Datasheet included with dilution recommendations, and related reagents. |
| Applications | WB/IHC-P/IF/ICC/IP/ELISA/ChIP |
| Host | Rabbit |
| Reactivity | Human/Mouse/Rat |
| Note | STRICTLY FOR FURTHER SCIENTIFIC RESEARCH USE ONLY (RUO). MUST NOT TO BE USED IN DIAGNOSTIC OR THERAPEUTIC APPLICATIONS. |
Product Properties
| Clonality | Polyclonal |
| Isotype | IgG |
| Conjugation | Unconjugated |
| Concentration | Lot specific |
| Purification | Affinity purification |
| Dilution Range | WB:1:500-1:2000IHC-P:1:50-1:200IF/ICC:1:50-1:200IP:0.5 Mu g-4 Mu g antibody for 200 Mu g-400 Mu g extracts of whole cellsELISA:Recommended starting concentration is 1 Mu g/mL. Please optimize the concentration based on your specif |
| Formulation | PBS with 0.02% Sodium Azide, 50% Glycerol, pH 7.3. |
| Storage Instruction | Store at-20°C for up to 1 year from the date of receipt, and avoid repeat freeze-thaw cycles. |
Target Information
| Gene Symbol | CBX5 |
| Gene ID | 23468 |
| Uniprot ID | CBX5_HUMAN |
| Immunogen Region | 1-191 |
| Immunogen Sequence | MGKKTKRTADSSSSEDEEEY VVEKVLDRRVVKGQVEYLLK WKGFSEEHNTWEPEKNLDCP ELISEFMKKYKKMKEGENNK PREKSESNKRKSNFSNSADD IKSKKKREQSNDIARGFERG LEPEKIIGATDSCGDLMFLM KWKDTDEADLVLAKEANVKC PQIVIAFYEERLTWHAYPED AENKEKETAKS |
| Specificity | Recombinant fusion protein containing a sequence corresponding to amino acids 1-191 of human HP1 alpha/HP1 alpha/CBX5 (NP_001120794.1). |
Additional Info
| Post Translational Modifications | Phosphorylation of HP1 and LBR may be responsible for some of the alterations in chromatin organization and nuclear structure which occur at various times during the cell cycle. Phosphorylated during interphase and possibly hyper-phosphorylated during mitosis. Ubiquitinated. |
| Function | Component of heterochromatin that recognizes and binds histone H3 tails methylated at 'Lys-9' (H3K9me), leading to epigenetic repression. In contrast, it is excluded from chromatin when 'Tyr-41' of histone H3 is phosphorylated (H3Y41ph). May contribute to the association of heterochromatin with the inner nuclear membrane by interactions with the lamin-B receptor (LBR). Involved in the formation of kinetochore through interaction with the MIS12 complex subunit NSL1. Required for the formation of the inner centromere. |
| Protein Name | Chromobox Protein Homolog 5Antigen P25Heterochromatin Protein 1 Homolog AlphaHp1 Alpha |
| Database Links | Reactome: R-HSA-4551638Reactome: R-HSA-8953750Reactome: R-HSA-983231Reactome: R-HSA-9843940 |
| Cellular Localisation | NucleusChromosomeCentromereColocalizes With Hnrnpu In The NucleusComponent Of Centromeric And Pericentromeric HeterochromatinAssociates With Chromosomes During MitosisAssociates Specifically With Chromatin During Metaphase And AnaphaseLocalizes To Sites Of Dna Damage |
| Alternative Antibody Names | Anti-Chromobox Protein Homolog 5 antibodyAnti-Antigen P25 antibodyAnti-Heterochromatin Protein 1 Homolog Alpha antibodyAnti-Hp1 Alpha antibodyAnti-CBX5 antibodyAnti-HP1A antibody |
Information sourced from Uniprot.org